摘要
核酸适体在治疗、诊断和生物传感等领域都引起了强烈的关注和广泛的应用。与传统的识别元素--抗体相比较,适体展现出很多的优点:尺寸小,化学性质稳定,容易制备和修饰。更重要的是适体在生物传感的设计上更为灵活,因此,产生了很多高选择性、高灵敏度的新型适体传感器。目前,很多的检测手段都被应用到适体传感器中,其中荧光的检测手段占有重要的地位。虽然荧光适体传感器已经取得了重大的进展,但是荧光标记给传感器的设计带来很多的不便,因此,免标记的荧光适体传感器备受关注。在本文中,我们将对免标记的荧光适体传感器的研究进展进行综述,为分析工作者发展更加灵敏、更加简单、更加应用广泛的免标记荧光适体传感器提供依据。
Nucleic-acid aptamers have received intense attention and found wide applications in a range of areas, such as thera- peutics, diagnostics and biosensors. In contrast to traditional antibodies, aptamers exhibit many advantages as molecular recognition ele- ments in biosensors： they are small in size, chemically stable, readily available and easily modified. More importantly, aptamers offer greater flexibility in biosensor design. Therefore, many high selectivity and high sensitivity aptasensors have been reported. To date, a va- riety of detection techniques have been used in aptasensors, among which fluorescence detection plays an important role. Although the fluorescence aptasensors have made significant progress, the fluorescence labeling for sensor design brings a lot of inconvenience, there-fore, label-free fluorescence aptasensors aroused general concern. In this paper, we summarized recent advances of the label-free fluores- cence aptasensors, providing the basis for the analysis of worker to development label-free fluorescence aptasensors, which are more sen- sitive, more simple and more widely used.
出处
《现代生物医学进展》
CAS
2015年第21期4194-4197,共4页
Progress in Modern Biomedicine
基金
黑龙江省教育厅科学技术研究项目(12511511)
关键词
适体
荧光
免标记
传感器
Aptamer
Fluorescence
Label-flee
Sensor
