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桂枝茯苓胶囊主要成分含量变化对主要药效学的影响研究 被引量:11

Effects on Content Changes of Main Components in Gui-Zhi Fu-Ling Capsule on Main Pharmacodynamics
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摘要 目的:观察桂枝茯苓胶囊主要成分含量变化对痛经模型大鼠、盆腔炎模型大鼠、子宫肌瘤模型大鼠的影响。方法:采用分子烙印技术定量逐级敲除桂枝茯苓胶囊中主要成分(敲除比例为10%、20%、30%、40%、50%、60%、70%、80%、90%),分别观察对缩宫素所致大鼠痛经模型的扭体次数、潜伏期、子宫组织中ET-1及PGF2α的影响;对混合菌液加机械损伤造成的慢性盆腔炎模型大鼠的TNF-α、IL-2的影响;对雌激素负荷法所致大鼠子宫肌瘤模型的子宫重量、子宫系数及血清中雌二醇(E2)、孕酮(P)水平的影响。结果:逐级敲除15个主要成分后药效逐渐降低,敲除10%-40%时,与模型比较仍有显著差异(P<0.01),而敲除50%-90%时,与模型组无明显差异(P>0.05),且药效比桂枝茯苓胶囊组明显降低(P<0.05或P<0.01)。结论:没食子酸、芍药苷、丹皮酚等15个化合物可能是桂枝茯苓胶囊发挥治疗作用的主要效应物质基础,同时也为桂枝茯苓胶囊主要成分的质控含量范围的制定提供了有益参考。 The rat model of dysmenorrhea, pelvic inflammation disease(PID), and uterine myoma were established to observe the effect of Gui-Zhi Fu-Ling Capsule(GZFLC) with the changes of main components. The molecular imprinted technology was used to quantitatively knock out main components in GZFLC(the knocked-out ratios were 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%). And the observation was made on the writhing times, latency, and effects on ET-1 and PGF2α of uterine tissues in the pitocin-induced dysmenorrhea rat model. Effects on TNF-α and IL-2 were observed in PID rat model induced by mixed bacteria plus mechanical damages. Effects on the weight and index of uterus, levels of E2 and P in serum were detected on the uterine myoma rat model induced by estrogen-burden. The results showed that the GZFLC efficacy was decreased with gradient knockout of 15 main components. When 10%-40% was knocked out, there was still significant difference compared to the model group(P〈0.01). However, when 50%-90% was knocked out, there was no significant difference compared to the model group(P〉0.05). Meanwhile, the GZFLC efficacy was decreased significantly compared to the GZFLC group(P〈0.05, or P〈0.01). It was concluded that 15 chemical compounds, which included gallic acid, paeoniflorin, paeonol, and etc., may be the main material basis of GZFLC. Meanwhile, it provided a useful reference for the quality control of main components in GZFLC.
出处 《世界科学技术-中医药现代化》 2015年第5期1066-1072,共7页 Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金 科学技术部重大新药创制项目(2013ZX09402203):重大新药创制-现代中药创新集群与数字制药技术平台 负责人:王振中
关键词 桂枝茯苓胶囊 分子烙印 痛经 子宫肌瘤 盆腔炎 Gui-Zhi Fu-Ling Capsule molecular imprinting dysmenorrhea uterine myoma pelvic inflammation disease
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