期刊文献+

Enhanced UV-B radiation during pupal stage reduce body mass and fat content, while increasing deformities, mortality and cell death in female adults of solitary bee Osmia bicornis

Enhanced UV-B radiation during pupal stage reduce body mass and fat content, while increasing deformities, mortality and cell death in female adults of solitary bee Osmia bicornis
原文传递
导出
摘要 The effects of enhanced UV-B radiation on the oogenesis and morpho- anatomical characteristics of the European solitary red mason bee Osmia bicornis L. (Hy- menoptera: Megachilidae) were tested under laboratory conditions. Cocooned females in the pupal stage were exposed directly to different doses (0, 9.24, 12.32, and 24.64 kJ/m2/d) of artificial UV-B. Our experiments revealed that enhanced UV-B radiation can reduce body mass and fat body content, cause deformities and increase mortality. Following UV exposure at all 3 different doses, the body mass of bees was all significantly reduced compared to the control, with the highest UV dose causing the largest reduction. Similarly, following UV-B radiation, in treated groups the fat body index decreased and the fat body index was the lowest in the group receiving the highest dose of UV radiation. Mortality and morphological deformities, between untreated and exposed females varied considerably and increased with the dose of UV-B radiation. Morphological deformities were mainly manifested in the wings and mouthparts, and occurred more frequently with an increased dose of UV. Cell death was quantified by the Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay (DNA fragmentation) during early stages of oogenesis of O. bicornis females. The bees, after UV-B exposure exhibited more germarium ceils with fragmented DNA. The TUNEL test indicated that in germarium, low doses of UV-B poorly induced the cell death during early development. However, exposure to moderate UV-B dose increased programmed cell death. In females treated with the highest dose of UV-B the vast majority of germarium cells were TUNEL-positive. The effects of enhanced UV-B radiation on the oogenesis and morpho- anatomical characteristics of the European solitary red mason bee Osmia bicornis L. (Hy- menoptera: Megachilidae) were tested under laboratory conditions. Cocooned females in the pupal stage were exposed directly to different doses (0, 9.24, 12.32, and 24.64 kJ/m2/d) of artificial UV-B. Our experiments revealed that enhanced UV-B radiation can reduce body mass and fat body content, cause deformities and increase mortality. Following UV exposure at all 3 different doses, the body mass of bees was all significantly reduced compared to the control, with the highest UV dose causing the largest reduction. Similarly, following UV-B radiation, in treated groups the fat body index decreased and the fat body index was the lowest in the group receiving the highest dose of UV radiation. Mortality and morphological deformities, between untreated and exposed females varied considerably and increased with the dose of UV-B radiation. Morphological deformities were mainly manifested in the wings and mouthparts, and occurred more frequently with an increased dose of UV. Cell death was quantified by the Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay (DNA fragmentation) during early stages of oogenesis of O. bicornis females. The bees, after UV-B exposure exhibited more germarium ceils with fragmented DNA. The TUNEL test indicated that in germarium, low doses of UV-B poorly induced the cell death during early development. However, exposure to moderate UV-B dose increased programmed cell death. In females treated with the highest dose of UV-B the vast majority of germarium cells were TUNEL-positive.
出处 《Insect Science》 SCIE CAS CSCD 2015年第4期512-520,共9页 昆虫科学(英文版)
基金 This work was supported by a Ministry of Science and Higher Education (N N311 298935) and University of Life Sciences (507.511.29) grants to O.W. and in part by a National Science Foundation Grant (EPS-0903787, MSU#269110-151250) grant to N.K. We would like to thank Agnieszka Graclik for her technical support in im- age analysis and preparation.
关键词 Osmia bicornis ovary development radiation TUNEL UV-B Osmia bicornis ovary development radiation TUNEL UV-B
  • 相关文献

参考文献32

  • 1Bosch, J. and Kemp, W.R (2000) Development and emer- gence of the orchard pollinator Osmia lignaria (Hymenoptera: Megachilidae). Environmental Entomology, 29, 8-13. 被引量:1
  • 2Blaustein, A.R., Romansic, J.M., Kiesecker, J.M. and Hatch, A.C. (2003) Ultraviolet radiation, toxic chemicals and am- phibian population declines. Diversity and Distributions, 9, 123-140. 被引量:1
  • 3Buck, N. and Callaghan, T.V. (1999) The direct and indirect effects of enhanced UV-B on the moth caterpillar Epirrita autumnata. Ecological Bulletins, 47, 68-76. 被引量:1
  • 4Caldwell, M.M. and Flint, S. (1994) Stratospheric ozone reduc- tion, solar UV-B radiation and terrestrial ecosystems. Cli- matic Change, 28, 375-394. 被引量:1
  • 5Caldwell, M.M., Bj6rn, L.O., Bornman, J.E, Flint, S.D., Ku- landaivelu, G., Teramura, A.H. and Tevini, M. (1998) Effects of increased solar ultraviolet radiation on terrestrial ecosys- tems. Journal of Photochemistry and Photobiology B: Biol- ogy, 46, 40-52. 被引量:1
  • 6Caldwell, M.M., Bornman, J.E, Balar6, C.L., Flint, S.D. and Kulandaivelu, G. (2007) Terrestrial ecosystems, increased ul- traviolet radiation, and interactions with other climate change factors. Photochemical and Photobiological Science, 6, 252 266. 被引量:1
  • 7Cockell, C.S. and Blaustein, A.R. (2000) 'Ultraviolet spring' and the ecological consequences of catastrophic impacts. Ecology Letters, 3, 77-81. 被引量:1
  • 8Daimon, T., Hirayama, Ch., Kanai, M., Ruike, Y., Meng, Y., Kosegawa, E., Nakamura, M., Tsujimoto, G., Katsuma, S. and Shimada, T. (2010) The silkworm Green b locus encodes a quercetin 5-O-glucosyltransferase that produces green co- coons with UV-shielding properties. Proceedings of the Na- tional Academy of Sciences of the United States of America, 107, 11471-11476. 被引量:1
  • 9Danks, H.V. (2004) The roles of insect cocoon in cold conditions. European Journal of Entomology, 101,433-437. 被引量:1
  • 10Fliszkiewicz, M., Giejdasz, K., Wasielewski, O. and Krishnan, N. (2012) Influence of winter temperatre and simulated cli- mate change on body mass and fat body depletion during diapause in adults of the solitary bee, Osmia rufa L (Hy- menoptera: Megachilidae). Environmental Entomology, 41, 1621-1630. 被引量:1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部