摘要
目的探讨PM2．5对哮喘急性期小鼠脾脏CD4^＋T淋巴细胞分化的影响。方法1．用卵蛋白（OVA）致敏和激发建立哮喘急性期小鼠模型；2．用智能总悬浮颗粒物（TSP）中流量采样器采集湛江市区PM2．5，制备PM2．5干粉；3．无特殊病原体（SPF）级6—8周龄雌性Balb／c小鼠64只，按随机数字表法随机分为阴性对照组（NC组）、哮喘对照组（AC组）、致敏小鼠不同剂量PM2．5处理组（SP组）及哮喘小鼠不同剂量PM2．5处理组（AP组）。SP组和AP组依据PM2．5剂量的不同各分3个亚组，每组8只。实验第0、7及14天致敏AC组、SP组和AP组，NC组用9g／L盐水腹腔注射作为对照；实验第17天用气管滴注法予SP1组／AP1组、SP2组／AP2组及SP3组／AP3组50μL低、中、高浓度的PM2．5混悬液，NC组和AC组滴注50μL 9g／L盐水作为对照；实验第21天至第23天AC组和AP组用OVA溶液雾化激发。末次激发24h后处死小鼠，无菌条件下取脾，流式细胞术（FCM）分别检测脾脏CD4^＋T淋巴细胞中调节性T淋巴细胞（Treg）、1型辅助性T淋巴细胞（Th1）、Th2及Th17的百分率。结果1．成功建立OVA诱导哮喘急性期小鼠模型；2．对比脾脏CD4^＋T淋巴细胞中Treg百分率显示：SP组[（12．28±0．73）％、（11．93±0．81）％及（11．70±1．14）％f及AC组f（12．18±1．00）％]较NC组[（13．50±0．39）％]降低（P〈0．05），AP3组[（10．58±0．65）％]较AC组及AP1组[（11．91±0．79）％]降低（P〈0．05）；3．对比脾脏CD^＋T淋巴细胞中Th1／Th2比值显示：SP1组[（7．74±1．21）％]较NC组[（5．52±1．06）％]升高（P〈0．05），SP2组[（6．30±0．58）％]较SP1组降低（P〈0．05），SP3组[（4．87±0．82）％]较SP2组降低（P〈0．05），AC组[（3．69±0．47）％]较NC组、SP3组降低（P〈0．05）；AP3组[（2．92±0．57）％]较AC组降低（P〈0．05）；4．对比脾脏CD4^＋T淋巴细胞中
Objective To evaluate the effects of PM2.5 on the differentiation of splenic CD4^ ＋ T lymphocytes in acute asthma mice. Methods （ 1 ） Mouse models of acute asthma were established by ovalbumin （OVA） sensitization and challenge. （2） PM2.5 was collected in the urban area of Zhanjiang city under heavy traffic and serious air pollution from total suspended particulate（TSP） mid - flow sampler and multistage particles cutters and the dry powder of PM2.5 was prepared. （3） Specific-pathogen free Balb/c mice, female, at 6 to 8 weeks of age were randomly divided into 8 groups （8 mice each group） ：a negative control group （NC group） , asthma control group （AC group） , sensitized mice treated with different doses of PM2.5 groups （ SP groups ） and asthmatic mice treated with different doses of PM2. 5 groups （AP groups）. SP groups and AP groups were respectively divided into 3 subgroups according to the dose of PM2.5. The AC group,SP groups and AP groups were sensitized on D0,D7 and D14,and the NC group was treated with NS as controls. The SP1/AP1 group, SP2/AP2 group and SP3/AP3 group were respectively given 50 ILL PM2.5 suspension. NC group and AC group were instilled with NS as controls. AC group and AP groups were challenged by aerosol of OVA, and NC group and SP group were treated with NS as controls. Twenty - four hours after last challenge, all the mice were sacrificed, and the percentage of regulatory T cells（ Treg）, T helper cell type 1 （Th1）, Th2 and Th17 of splenic CD4 ＋ T lymphocytes was detected by flow cytometry （FCM）. Results （ 1 ） An OVA - induced mouse models with acute asthma were successfully established. （ 2 ） Comparison of the percentage of Treg of splenic CD4 ^＋ T lympho- cytes：SP group[ （12.28 ±0.73）% ,（11.93 ±0.81）% and （11.70 ± 1.14）% ] and AC group[ （12.18 ± 1.00）% ] were lower than that in the NC group[ （13.50 ±0.39）% ] （P 〈0.05） ,AP3 group[ （ 10.58 ±0.65）% ] was lower than that in the AC group a
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2015年第16期1269-1272,共4页
Chinese Journal of Applied Clinical Pediatrics
基金
广东省科技厅课题（20138021800077）
