摘要
目的研究糖皮质激素治疗重症肌无力(MG)患者外周血CD4+CD25+调节性T细胞(Treg)中Foxp3和胞内CTLA-4的表达水平的变化。方法收集MG患者34例,分为非治疗组8例和糖皮质激素治疗组26例。另将20例健康者设为对照组。采用流式细胞仪检测分析患者和健康者外周血CD4+CD25+Treg细胞Foxp3和胞内CTLA-4的变化。结果 1 MG非治疗组患者外周血CD4+CD25+Treg细胞Foxp3和胞内CTLA-4水平较对照组显著下降(P<0.05);2糖皮质激素治疗能显著提升CD4+CD25+Treg细胞Foxp3和胞内CTLA-4表达比例(P<0.05),但仍低于对照组(P<0.05);3 CD4+CD25+Treg细胞Foxp3和胞内CTLA-4表达呈正相关性(r=0.870,P<0.001)。结论 MG患者外周血CD4+CD25+Treg Foxp3和胞内CTLA-4呈低表达,糖皮质激素治疗能显著提升Foxp3和胞内CTLA-4表达水平,但尚未达正常者免疫状态。
Objective To study the expression alteration of Foxp3 and intracellular CTLA-4 of CD4 ＋ CD25＋ regula- tory T cells in peripheral blood of myasthenia gravis patients under glucocorticoids treatment. Methods 34 patients with myasthenia gravis were selected, and subgrouped with no treatment group （8 cases） or glucocorticoids treat- ment group（26 cases）. Another 20 healthy subjects were set as the control group. 6-color flow eytometry was used to analyze the expression of Foxp3 and intraeellular CTLA-4 of CD4＋ CD25＋regulatory T cells in their peripheral blood of participants. Results （1） Compared with the control group, the level of Foxp3 and intracellular CTLA-4 of CD4＋ CD25 ＋ regulatory T cells in peripheral blood of in no treatment group were significantly decreased （P 〈 0.05 ）. （2） the level of Foxp3 and intraeellular CTLA-4 of CD4＋ CD25＋ regulatory T cells in peripheral blood of pa- tients with glucocortieoids treatment group were significantly higher than that of no treatment group （ P 〈 0. 05 ）, but were still lower than that of the control group （P 〈 0. 05 ）. （3） Foxp3 expression was positively correlated with intra- cellular CTLA-4 expression in CD4＋ CD25＋ regulatory T cells of patients with myasthenia gravis （ r = 0. 870, P 〈 0. 001 ）. Conclusion Low expression of Foxp3 and intracellular CTLA-4 of CD4＋ CD25＋ regulatory T cells in pe- ripheral blood is present in patients with sion of Foxp3 and intracellular CTLA-4, myasthenia gravis, glucocorticoids could significantly increase the expres- however, which still do not reach the immune state of healthy subjects.
出处
《安徽医科大学学报》
CAS
北大核心
2015年第9期1297-1300,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省青年科学基金项目(编号:11040606Q08)
