摘要
目的:观察丹参提取物对大鼠心肌梗死后心肌组织血管新生的作用并分析其可能的机制。方法:采用经典的冠状动脉左前降支结扎造模方法成功复制大鼠心肌梗死模型后,随机将大鼠分为模型组以及丹参提取物低(10 mg·kg-1·d-1)、中(20 mg·kg-1·d-1)、高(40 mg·kg-1·d-1)剂量治疗组,另设假手术组大鼠为对照组,各组大鼠数量均为8只。丹参提取物各治疗组给予上述对应的各药物剂量灌胃给药,模型组及对照组大鼠给予等量的生理盐水灌胃,连续饲养4周后应用HE染色、Masson染色和电镜法观察大鼠左心室心肌组织和血管结构病理成分变化,免疫组化染色分析心肌组织中血管内皮生长因子(VEGF)及CD34蛋白的表达变化。结果:组织病理学分析表明,与假手术组相比,模型组大鼠心肌组织形态较为紊乱,部分心肌细胞轮廓消失,坏死心肌组织呈现明显的纤维化,血管不完整,血管超微结构模糊或者消失;丹参提取物治疗之后,新生的血管数量明显增多,且超微结构分析表明,内皮细胞形态相对完整。心肌组织中VEGF及CD34蛋白表达结果证实,模型组较假手术组大鼠表达略有增加,但没有统计学差异;和模型组比较,丹参提取物各治疗组VEGF及CD34蛋白表达明显增多(P<0.01)。结论:丹参提取物可明显促进大鼠心肌梗死后心肌组织的血管新生。
AIM： To determine the effect of salvia extract on angiogenesis of the myocardium in the rats with myocardial infarction（ MI） and to analyze its possible mechanism. METHODS： Left coronary artery of Sprague-Dawley rats was ligated to establish a MI model. The rats were randomly divided into MI model group,3 different dose groups of salvia（ 10,20 and 40 mg·kg- 1·d- 1）,and sham operation group. Each group consisted of 8 rats. The rats in all treatment groups were orally administered with the salvia extract,and the rats in MI group and sham operation group were fed with the same volume of saline. The rats were sacrificed 4 weeks later. The hemodynamic changes of the rats were determined,and the segmental heart samples were used for morphological observation by hematoxylin and eosin staining,Masson staining,or electron microscopic analysis. The expression of vascular endothelial growth factor（ VEGF） and cluster of differentiation 34（ CD34） was analyzed according to immunohistochemistry. RESULTS： Compared with sham operation group,the morphological changes of the myocardium in MI group were disordered,part of myocardial cell outline disappeared,and obvious fibrosis in the necrosis myocardial tissue and fuzzy or disappearing microvascular ultrastructure were also observed. Compared with MI group,the number of new microvessels in all the treatment groups increased obviously,and the morphological changes of the endothelial cells were relatively complete according to electron microscopy. Compared with sham operation group,the protein expression of VEGF and CD34 in the cytoplasm of the myocardial tissues in MI group increased only a little. Compared with MI group,the protein expression of VEGF and CD34 in the cytoplasm of the myocardial tissues in all treatment groups increased significantly（P〈0. 01）. CONCLUSION： Salvia extract obviously promotes angiogenesis of the myocardial tissues in the rats after myocardial infarction.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2015年第8期1490-1494,1499,共6页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.81202791
No.81473438
No.81173372)
河南省中医内科学重点学科支撑课题(豫教高[2012]186号)
关键词
丹参
心肌梗死
血管新生
内皮细胞
Salvia
Myocardial infarction
Angiogenesis
Endothelial cells
