摘要
目的:研究丹参提取物对血管紧张素Ⅱ(AngⅡ)致心肌肥大及相关基因表达的影响,并探讨其抑制心肌肥大的作用机制。方法:乳鼠心肌细胞原代培养,复制AngⅡ致心肌细胞肥大模型,随机分为模型组(10-6g.L-1AngⅡ),缬沙坦(10-4,10-3,10-2g.L-1Val)对照组,丹参提取物10-4,10-3,10-2g.L-1组的丹参提取物。BI-2000医学图像分析系统进行心肌细胞计数和直径测定,Bradford法测定心肌细胞蛋白质含量,反转录PCR(RT-PCR)法测定蛋白激酶D1(PKD1)mRNA的表达。结果:与正常对照组相比,模型组心肌细胞数量上变化不大,心肌细胞直径、总蛋白质含量及PKD1 mRNA的表达明显增加(P<0.05);和模型组相比,Val对照组心肌细胞直径、总蛋白质含量及PKD1 mRNA的表达明显降低(P<0.05);而丹参提取物中、高剂量组心肌细胞直径、总蛋白质含量及PKD1 mRNA的表达均明显降低(P<0.05)。结论:丹参提取物能显著抑制Ang II诱导的心肌细胞肥大,可能与对PKDl mRNA的表达调控密切相关。
Objective： To explore the inhibition effect of salvia extract on cultured neonatal rat hypertrophic cells induced by angiotensin Ⅱ （Ang Ⅱ ） and the related gene regulation to mRNA expression. Method： The Ang Ⅱ-induced cardiac myocyte hypertrophy model was replicated, the neonatal rat cardiomyocytes in primary culture were randomly divided into normal control group, model group, valsartan （Val） in the control group, and salvia extract three different dose groups. There was any added drugs in the normal control group, and the final concentration was 10 -6 g.L - x Ang ]1 in the model group, 10 -6 g.L - 1 Val iff Val control group, and 10 -4, 10-3, 10-2g.L-1 salvia extract in the other three groups. Myocardial ceil counting and diameter were determined by the BI-2000 medical micrograph analyzing system, protein content was detected by the Bradford method, and protein kmase D1 （PKD1） mRNA expression was analysed by the reverse transcription PC R （RT-PCR） method. Result： Compared with the normal control group, the number of myocardial ceils in model group changed little, yet cardiomyocyte diameter, total protein content and PKD1 mRNA expression were significantly increased （P 〈 0.05）; compared with the model group, cardiomyocyte diameter, the total protein content and PKD1 mRNA expression of the Val control group were significantly lower （P 〈 0.05） ; as well as the 10-3, 10-2g.L-1 salvia extract groups （P 〈 0.05）. Conclusion： Salvia extract can significantly inhibit hypertrophy of cardiac myocyte induced by Ang Ⅱ, which may be closely related to the mRNA expression regulation of PKD1.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第2期263-266,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金面上项目(81173372)
