摘要
目的建立一种基于毛细管电泳-化学发光联用法检测粮食中伏马菌素B1的快速检测方法。方法基于伏马菌素B1对鲁米诺-三价银[Ag（Ⅲ）]化学发光体系有抑制作用,结合毛细管电泳分离技术,对检测方法进行优化,选择5×10-3mol/L硼砂为电泳缓冲溶液,鲁米诺浓度为2×10-3mol/L,Ag（Ⅲ）浓度为3×10-5mol/L溶解于0.01 mol/L Na OH。结果经过条件优化后进行试验,伏马菌素B1的线性范围为1~200μg/ml,检出限（S/N=3）为1μg/ml。对200μg/ml的伏马菌素B1进行8次平行测定,其出峰时间和峰高的相对标准偏差分别为2.64%和7.86%。结论该方法操作简便、快速、准确可靠,可用于伏马菌素B1的检测,适用于玉米中伏马菌素B1的测定,结果比较理想。
Objective The purpose of this research is to establish and optimize a new method for accurate and rapid capillary electrophoresis-chemiluminescence detection of fumonisin B1. Methods The conditions for luminescence were optimized based on the effect of fumonisin B1 on the chemiluminescence system of Ag（ Ⅲ ） and lumonol. The buffer solution was 2 ×10^-3mol/L luminol solution containing 5× 10^-3 mol/L borax, and the oxidation reagent was the 3 × 10^5 mol/L Ag（ Ⅲ ） solution containing sodium hydroxide. Results After optimization of separation and detection, the linear range was from 1 to 200μg/ml, and the detection limit （S/N = 3） was 1 μg/ml. On the eight parallel detections of 200 μg/ml fumonisin B1 , the relative standard deviations of peak time and peak height were 2.64% and 7.86%. Conclusion The method is simple, rapid, accurate and reliable for the detection of fumonisin B1, and could be applied to detect fumonisin B. in corn.
出处
《中国食品卫生杂志》
2015年第4期382-385,共4页
Chinese Journal of Food Hygiene
基金
国家自然科学基金项目(81373046
81402723)
