摘要
目的观察术前不同时长睡眠剥夺(sleep deprivation,SD)对大鼠术后认知功能和海马胆碱乙酰转移酶(choline acetyl transferase,ChAT)表达的影响。方法 40只14个月龄Wistar大鼠,雌雄各半,随机分为四组:正常对照组(C组)、D1、D2、D3组,每组10只。D1、D2、D3组分别术前剥夺睡眠1、2、3d。四组大鼠均进行Morris水迷宫训练。C组于第五天行脾切除手术,D1、D2、D3组行睡眠剥夺,结束后对大鼠行脾切除手术。术后24h进行水迷宫测试,免疫组化法测定海马ChAT阳性细胞的表达。结果与C组比较,术后D1、D2、D3组逃避潜伏期及游泳距离明显延长、ChAT的表达明显减少(P<0.05)。D1、D2、D3组随着SD时间的延长,逃避潜伏期和游泳距离明显延长(P<0.05),ChAT的表达明显减少(P<0.05)。结论术前睡眠剥夺可引起大鼠术后空间学习记忆功能减退,且时间越长功能减退越明显。
Objective To observe the influences of preoperative sleep deprivation with different length on postoperative cognitive dysfunction and the expression of hippocampus acetylcholine trans ferase in rats. Methods Forty 14-month-old Wistar rats, including 20 male and 20 female, were randomly divided into control group （group C, n= 10）, and 3 sleep deprivation groups （group D1, D2, D3, received preoperative sleep deprivation for 1, 2 or 3 days respectively, n= 10 for each group）. Rats received Morris water maze training one time per day for 4 consecutive days. Then splenectomy was performed on the fifth day. 24 hours after operation rat received Morris water maze test again. Then the expression of ChAT （Choline acetyl transferase） positive cells in hippocampus was test by immunohistochemical methods. Results Compared with group C, the escaping latency and the swimming distance in all sleep deprivation groups was significantly extended, meanwhile the expression of ChAT was decreaesd （P〈0.05）. The longer SD time, the longer escaping latency, the longer swimming distance and the lower expression of ChAT in all sleep deprivation groups （P〈 0.05 ）. Conclusion Preoperative sleep deprivation could impair postoperative spatial learning and memory function in rats, and the more longer SD time is, the more obvious POCD is.
出处
《临床麻醉学杂志》
CAS
CSCD
北大核心
2015年第8期790-792,共3页
Journal of Clinical Anesthesiology
