摘要
在中温条件下进行实验室规模的连续式餐厨垃圾厌氧消化试验。采用实时荧光定量PCR（RT-PCR）技术对餐厨垃圾单相厌氧消化失衡条件下系统内古菌和产甲烷菌的数量变化规律进行探析。结果表明,在厌氧消化系统从稳定期到抑制期的过程中,古菌和产甲烷菌的基因浓度都明显降低,其降幅达到70%~80%。2次冲击负荷试验过程中,产甲烷菌基因浓度降幅都大于古菌,说明在系统抑制过程中产甲烷菌相对于古菌来说受到的影响更大。当厌氧消化系统出现抑制状态时,产甲烷菌不能适应环境变化而大量死亡,导致系统平衡被破坏。
Lab-scale continuous anaerobic digestion of food waste at mesophilic temperature was carries out in this study. RT-PCR method was used to analyze the microbial diversity of sludge during every stage in the reactor. In the process of anaerobic digestion system from the stable stage to the inhibition stage,the DNA concentration of both archaea and methanogen bacteria reduced drastically with a decline of 70% to 80%. In the two shock load tests from stable stage to inhibition stage, the decline of methanogen bacteria concentration was higher than archaea, which showed that in the process of anaerobic digestion system methanogen bacteria in archaea got greater impact than other archaeas. When there was inhibition of anaerobic digestion system, methanogen bacteria couldn＇t adapt to the anaero- bic system within the changing environment and a large number of death, the balance of anaerobic digestion system was destroyed.
出处
《环境污染与防治》
CAS
CSCD
北大核心
2015年第8期26-30,共5页
Environmental Pollution & Control
基金
云南农业大学科研启动基金资助项目
