摘要
目的:建立利用近红外光谱技术快速测定制何首乌中水分的方法。方法:烘干法测定135批制何首乌中的水分,采用标准归一化法,二阶导数法对近红外光谱进行预处理,结合偏最小二乘法建立何首乌中水分的定量分析模型。结果:所建立的模型,内部交叉验证系数达到0.990 71,校正均方差为0.152,预测均方差为0.175,内部交叉验证均方差为0.482 57。结论:该方法方便、准确、可靠、重复性好,可快速测定制何首乌中的水分。
Objective： To establish a method for determination of the moisture in Polygoni Multiflori Radix Praeparata by near-infrared spectroscopy（ NIRS）. Method： The moisture content of 135 samples was determined by the method of drying under oven. The second derivative and the standard normal variate（ SNV）were used as spectral preprocessing options,and calibration model of moisture content was established by the partial least squares regression analysis. Result： The correlation coefficients,the root-mean-square error of calibration,the root-mean-square error of predication and the root-mean-square error of cross-validation of the calibration model for moisture content were 0. 990 71,0. 152,0. 175 and 0. 482 57. Conclusion： The method is convenient,accurate,reliable and it is of high reproducibility and can predict moisture content of Polygoni Multiflori Radix Praeparata rapidly.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第16期52-55,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省科技计划项目(2009B030801044)
关键词
制何首乌
水分
近红外光谱
Polygoni Multiflori Radix praeparata
moisture content
NIRS
