摘要
采用共滴定法、溶胶一凝胶法、冷冻干燥法并以氨水为交联剂制备了纳米HAP／COL／KGM多孔复合骨支架，利用IR、XRD、SEM、TEM及EDS对所制备的粉体和骨支架进行分析表征，根据有关标准对骨支架进行了平均孔径、抗压强度、体外降解、干细胞相容性实验、动物皮下植入实验。结果表明，共滴定法所制备的粉体为10-100nm HAP／COL复合纳米粉，最终获得的骨支架为三维多级连通孔网络结构；随着氨水量即交联度增加骨支架平均孔径和抗压强度增大并分别达到350μm和3MPa，随着氨水量增加支架降解率减小；骨支架有利于兔BMSCs黏附增殖生长，干细胞相容性良好；体内大量细胞能黏附在骨支架表面并迁移到支架内部中心且生长良好，支架有较好组织相容性和体内降解性。
The nano HAP/COL/KGM porous composite bone scaffolds were prepared by simultaneous titration method, sol-gel method and freeze-drying method, using ammonia as a crosslinking agent. The prepared powders and the bone scaffolds were characterized by IR, XRD, SEM, TEM and EDS. The experiments of average pore diameter, compressive strength, degradation in vitro, stem cell compatibility and animal subcutaneous implantation were carried out according to the related standard. The results show that the obtained powders are the HAP/COL composite nanometer powders sized by 10-100 nm by the method of simultaneous titration and the finally obtained bone scaffolds appear three-dimension network structure with hierarchically interconnected pore; with the increase of the content of ammonia namely the increase of the crosslinking degree, the average pore diameter and the compressive strength of the bone scaffolds increase and can respectively reach 350 μm and 3 MPa but the degradation rate decreases; the bone scaffolds are beneficial to the adhesion, proliferation and growth of rabbit BMSCs and have good stem cell compatibility; a large number of cells in vivo can adhere to the surface of the bone scaffolds and migrate into the center of the bone scaffolds and grow well and the bone scaffolds have good histocompatibility and degradation in vivo.
出处
《人工晶体学报》
EI
CAS
CSCD
北大核心
2015年第7期1961-1967,共7页
Journal of Synthetic Crystals
基金
国家自然科学基金(31260228
81260272)
云南省自然科学基金国际合作项目(2011IA008)
