摘要
目的探讨microRNA（miRNA）在间变性淋巴瘤激酶（ALK）阴性的问变性大细胞淋巴瘤和CD30阳性非特殊型外周T细胞淋巴瘤中表达的差异性及miRNA在ALK阴性的间变性大细胞淋巴瘤发病机制中的作用。方法应用高通量miRNA芯片技术对3例原发于淋巴结的ALK阴性的间变性大细胞淋巴瘤，3例原发于淋巴结的CD30阳性非特殊型外周T细胞淋巴瘤和3例反应性增生的淋巴结进行检测；筛选出7种重要miRNA，应用即时定量聚合酶链反应方法检测这7种miRNA在15例原发于淋巴结的ALK阴性的间变性大细胞淋巴瘤和15例原发于淋巴结的CD30阳性非特殊型外周T细胞淋巴瘤中的表达。结果miRNA表达谱分析显示，ALK阴性的间变性大细胞淋巴瘤和CD30阳性非特殊型外周T细胞淋巴瘤两组之间差异有统计学意义（P〈0．05）的miRNA有13种。其中5种miRNA验证结果与表达谱结果差异性表达趋势一致：miR-664b-5p、miR-1275、miR-4739、miR-4736和miR-504-5p，表达差异有统计学意义（P值分别为0．004、0．021、0．031、0．009和0．007）。与反应性增生的淋巴结相比在ALK阴性的间变性大细胞淋巴瘤中miR-664b-5p、miR-1275、miR-4739为下调表达，miR-4736和miR-504-5p为上调表达。结论检测miR-664b-5p、miR-1275、miR-4739、miR-4736和miR-504-5p有助于鉴别ALK阴性的间变性大细胞淋巴瘤和CD30阳性的非特殊型外周T细胞淋巴瘤。miR-4739、miR-4736和miR-1275可能通过靶基因TNFRSF8和TMODl在ALK阴性的间变性大细胞淋巴瘤的发病中起重要作用。
Objective To study the role of microRNAs （miRNAs） in ALK-negative anaplastic large cell lymphoma and CD30 positive peripheral T cell lymphoma （not otherwise specified） , and discuss the pathogenesis of miRNAs in ALK-negative anaplastic large cell lymphoma. Methods Three cases of ALK-negative anaplastic large cell lymphoma of lymph node, 3 cases of CD30-positive peripheral T cell lymphoma （ not otherwise specified） of lymph node and 3 cases of reactive hyperplasia of lymph node were detected by high flow microarray of miRNAs. The method of real-time quantitative polymerase chain reaction was further applied for 7 miRNAs in 15 cases of ALK-negatie anaplastic large cell lymphomas of lymph node and 15 cases of CD30-positive peripheral T cell lymphoma （not otherwise specified） of lymph node. Results The significant difference of 13 miRNAs was found between ALK-negative anaplastic large cell lymphoma and CD30 positive peripheral T cell lymphoma （ not otherwise specified） （ P 〈 0. 05 ） , of which the result of 5 miRNAs was consistent with miRNAs expression spectrum ： miR-664b-Sp, miR-1275, miR-4739, miR-4736 and miR-504-Sp, the difference was statistically significant （P 〈 0. 05 ）. Compared with reactive hyperplasia of lymph nodes, miR-664b-Sp, miR-1275 and miR-4739 were significantly under-expressed （P = 0. 004, P =0. 021, P =0. 031 ） and miR-4736 and miR-504-Sp were significantly over-expressed （P =0. 009, P = 0. 007） in ALK negative anaplastic large cell lymphoma. Conclusions MiR-664b-5p, miR-1275, miR-4739, miR-4736 and miR-504-5p may become an important indicator in the differentiation ALK-negative anaplastic large cell lymphoma from CD30-positive peripheral T cell lymphoma （ not otherwise specified）. MiR-4739, miR-4736 and miR-1275 may play important role in pathogenesis of negative- anaplastic large cell lymphoma by target genes： TNFRSF8 and TMOD1.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
2015年第8期565-570,共6页
Chinese Journal of Pathology
基金
福建省卫生厅青年科研课题基金（2012-1-2）
