摘要
目的研究美藤果油对非酒精性脂肪性肝炎(NASH)大鼠的保护作用。方法采用高脂饲料饲喂SD大鼠4周,建立NASH模型,设置空白组,NASH模型组,脂肪性肝炎对照组,紫苏油对照组,美藤果油高(1.6 m L/kg)、中(0.8 m L/kg)、低(0.4 m L/kg)剂量组,每组12只大鼠。给药45 d后,检测各组大鼠血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平,并使用HE染色法观察比较各组的肝脏病理情况,进行统计比较。结果模型组的TC、TG、LDL-C水平依次为(1.295±0.143)、(0.738±0.095)、(0.194±0.019)mmol/L;美藤果油中剂量组的TC、TG、LDL-C水平依次为(0.610±0.031)、(0.402±0.020)、(0.136±0.005)mmol/L;美藤果油高剂量组的TC、TG、LDL-C水平依次为(0.662±0.036)、(0.436±0.029)、(0.147±0.011)mmol/L;相对于模型组,美藤果油中、高剂量组的TC、TG、LDL-C水平均下降(P<0.05或<0.01)。相对于模型组,美藤果油中、高剂量组对ALT、AST、TNF-α、IL-6水平均有下调作用(P<0.05或<0.01),且对NASH大鼠的肝脏病理改变有改善作用(P<0.01)。结论美藤果油对NASH大鼠具有一定的保护作用,可能主要通过降低血脂水平和抑制炎症细胞因子而实现。
Objective To study the protective effect of Saeha Inhi oil on non-alcoholic steatohepatitis （NASH） rats. Methods A rat model of NASH was established by high fat diet for 4 weeks. Blank control group of normal diet, NASH model group, steatohepatitis control group, perilla oil control group, and Sacha Inhi oil groups with high （ 1.6 mL/kg）, middle （0.8 mL/kg）, and low （0.4 mL/kg） dosages were set up. After 45 d, levels of total cholesterol （ TC）, triglyceride （ TG）, high density lipoprotein cho- lesterol （ HDL-C）, low density lipoprotein cholesterol （ LDL-C ）, alanine aminotransferase （ ALT）, aspar- tate transaminase （AST）, interleukin-6 （IL-6）, and tumor necrosis factor-α （TNF-α） were tested. Changes of pathologic histology in the liver were observed by HE staining. Then the statistical comparison was conducted. Results Levels ofTC, TG, and LDL-C were （1.295 ±0. 143）, （0.738 ±0.095）, and （0.194±0.019） mmol/L in the model group, （0. 610 ± 0. 031）, （0. 402 ± 0.020）, and （0.136±0.005） mmol/L in the middle-dose Sacha Inhi oil group, and （0. 662 ± 0. 036 ）, （0.436 ±0.029）, and （0. 147 ±0. 011 ） mmol/L in the high-dose Sacha Inhi oil group, respectively. Compared with model group, levels of TC, TG, and LDL-C in the middle- and high-dose Sacha Inhi oil groups were lowered （P 〈 0.05 or P 〈 0.01 ）. Compared with the model group, the middle- and high-dose Sacha Inhi oil groups can lower the levels of AST, ALT, IL-6, and TNF-α （P 〈0.05 or P 〈0. 01 ） and improve the changes of pathologic histology in liver of NASH rats （ P 〈 0.01 ）. Conclusion Sacha Inhi oil has a certain protective effect on NASH rats, which probably works mainly through lowering blood lipid lev- el and inhibit inflammatory cytokines.
出处
《华南预防医学》
2015年第4期342-346,共5页
South China Journal of Preventive Medicine
关键词
美藤果油
非酒精性脂肪性肝炎
血脂
转氨酶
炎症细胞因子
Sacha Inchi oil
Non-alcoholic steatohepatitis
Blood lipid
Aminotransferase
Inflammatory cytokines
