摘要
目的:为了提高齐墩果酸的生物利用度和靶向性,以卵磷脂和胆固醇为载体,研究齐墩果酸脂质体制备方法和制备工艺,并建立包封率测定方法。方法:采用薄膜超声分散法制备脂质体,高效液相色谱法测定齐墩果酸的含量。以包封率为评价指标,采用正交试验法优选处方和工艺。结果:胆固醇、卵磷脂、齐墩果酸的最佳比例为2∶10∶1,制备温度为45℃,制得的脂质体包封率为87.85%。结论:该工艺方法简便、稳定可行,适用于齐墩果酸脂质体的制备。
Objective： To improve bioavailability and targeting property of oleanolic acid, lecithin and cholesterol were used as carrier materials to optimize prescription and preparation technology of oleanolic acid liposome, and the method of entrapment efficiency determination was established. Methods： The liposome was prepared by thin-film ultrasonic dispersion method, and the contents of oleanolic acid were determined by HPLC. With the entrapment efficiency as evaluation index, prescription and process were optimized by orthogonal test. Results： The optimum weight ratio of cholesterol to lecithin and oleanolic acid was 2∶10∶1, and the temperature of preparation was 45 ℃. The entrapment efficiency was 87.85 %. Conclusion： The process was feasible, stable and applicable for the production of oleanolic acid liposome.
出处
《广东化工》
CAS
2015年第13期18-19,共2页
Guangdong Chemical Industry
基金
广东省教育厅学科建设专项资金(2013KJCX0110)
广东药学院大学生创新训练项目(2013)
关键词
齐墩果酸
脂质体
制备工艺
包封率
Oleanolic acid
liposome
preparation process
entrapment efficiency
