摘要
背景:近年在整体水平上以高通量分子扫描手段为基础的基因组学、蛋白质组学以及计算机辅助设计等技术的整合及相互关联的"技术链"的应用已在乳腺癌、肺癌、胃癌、结肠癌、卵巢癌、黑色素瘤等的研究中取得了丰硕的成果,但关于口腔鳞状细胞癌的研究较少。目的:实验通过基因表达谱芯片检测口腔鳞状细胞癌组织与癌旁正常组织的基因表达谱。方法:收集广东省口腔医院2013年手术切除的口腔鳞癌及癌旁正常组织各2例,采用Roche Nimble Gen全基因组表达谱芯片进行口腔鳞癌及癌旁正常组织的基因表达谱检测。结果与结论:按差异基因筛选标准,从32 448条检测基因中筛选出口腔鳞癌肿瘤组织的差异基因共有7 872条,占筛选基因总数的24%;其中上调表达的基因有3 800条,下调表达的有4 072条。结果证实,通过基因表达谱芯片检测并根据表达差异1倍以上的筛选标准得到了7 872个表达差异的基因。由此可见肿瘤的发生发展不是单个或几个基因的作用结果,以往实验往往针对某个或某几个基因的研究有很大的局限性。同时也说明了肿瘤的产生是多基因成网络相互调节作用的结果,而且这个网络的作用关系是非常复杂的。
BACKGROUND： In recent years, based on high-throughput molecular imaging, integration of genomics, proteomics and computer aided design and the application of correlative ＂technical chains＂ have achieved great achievements in the research of breast cancer, lung cancer, gastric cancer, colon cancer, ovarian cancer and melanin tumor. However, there are few researches on oral squamous cell carcinoma. OBJECTIVE： To detect the gene expression profile of the oral squamous cell carcinoma tissue and normal paracarcinoma tissue using DNA chip-based gene expression profile. METHODS： Two samples of oral squamous cell carcinoma tissue and normal paracarcinoma tissue of patients who received treatment at Stomatological Hospital of Guangdong Province of China in 2013 were included in this study. The gene expression profiles of oral squamous cell carcinoma and normal paracarcinoma tissue were determined by the Roche Nimble Gen gene expression microarrays.RESULTS AND CONCLUSION： According to screening criteria of differential genes, 7 872 out of 32 448 detected genes were differentially expressed genes of oral squamous cell carcinoma, which accounts for 24% of the total number of the screening genes. 3 800 genes were up-regulated, and 4 072 were down-regulated. The results confirm that through detection with the help of gene expression profile clip, 7 872 differentially expressed genes were obtained through DNA chip-based gene expression profiles according to the screening criteria. Thus it can be concluded that the occurrence and development of the tumors are not a result of single or several genes. Previous experiments based on a single or several genes have great limitations. These findings also suggest that the occurrence of tumor is a result of mutual regulatory effects of many genes forming a network, moreover, the interactions of the network is quite complicated.
出处
《中国组织工程研究》
CAS
北大核心
2015年第27期4365-4370,共6页
Chinese Journal of Tissue Engineering Research
基金
广东省医学科学研究基金资助项目(A2011092)
国家自然科学基金资助项目(81271159/H1405)~~
