摘要
目的检测原发性布加综合征（BCS）患者外周血内皮祖细胞（EPCs）数量及功能变化，探讨BCS可能的发病机制。方法选取原发性BCS患者82例及健康对照者20例，采用流式细胞术检测受试者外周静脉血单个核细胞中CD34、CDl33和血管内皮生长因子受体2（KDR）阳性水平。密度梯度离心法分离、培养外周血单个核细胞，采用免疫荧光DiI—Ac-LDL和FITC—UEA-1双染色进行阳性细胞鉴定。采用MTF比色法、黏附能力测定和Transwell实验分别检测外周血EPCs的增殖、黏附和迁移能力。结果与健康对照组比较，BCS患者循环EPCs（CD34＋／CD133＋／KDR＋）的数量[（0．038±0．007）％比（0．020±0．005）％]、增殖能力（0．58±0．07比0．20±0．04）、黏附能力（35．0-i-2．5比15．8±1．6）、迁移能力（23．9±2．6比16．1±1．7）明显降低，差异均有统计学意（P〈0．05）。结论原发性BCS患者循环EPCs数量减少，功能下降，可能是其血管病变的重要因素之一。
Objective To evaluate the changes in the number and activities of endothelial progeni- tor cells （EPCs） from peripheral blood in patients with primary Budd-Chiari syndrome （BCS）, and to ex- plore the possible mechanisms of BCS. Methods Eighty-two patients with BCS and 20 healthy subjects used as eontrols were reeruited for this study. The EPCs from peripheral blood were counted by flow cytome= try for CD34, CD133 and KDR for positivity. The peripheral blood mononuelear cells were isolated by density gradient centrifugation and cultured for 7 days. Characterization of EPCs as adherent cells was done using double staining of FITC-UEA-1 and DiI-Ac-LDL binding. The proliferation, adhesion and migration activities were assayed by MTT chromatometry, adhesion activity assay and Transwell assay, respeetively. Results EPCs （CD34 ＋/CD133 ＋/KDR＋） were depleted in the BCS patients as compared to the healthy controls [ （0. 020 ±0. 005） % vs （0. 038 ±0. 007） % 1. The proliferation activities （0.20 ±0.04 vs 0.58 ±0.07）, adhesion activities （ 15.8 v 1.6 vs 35.0 ± 2.5 ） and migration activities （ 16.1 ± 1.7 vs 23.9 ± 2.6） were significantly lower in the BCS group than the control group （ P 〈 0.05 ）. Conclusion EPCs from the pe- ripheral blood in patients with BCS exhibited reduced numbers and impaired proliferation, adhesion and mi- gration activity, which may be the key factors for vasculopathy formation in primary BCS patients.
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2015年第7期466-469,共4页
Chinese Journal of Hepatobiliary Surgery
基金
江苏省卫生计生委课题（H201425）
关键词
布加综合征
内皮祖细胞
干细胞
Budd-Chiari syndrome
Endothelial progenitor cells
Stem cells
