摘要
目的:优选果胶酶法提取棉花根多糖的工艺。方法:利用果胶酶水解与传统热水浸提相结合的方法提取棉花根多糖,以多糖提取率为评价指标,结合单因素试验和正交设计,考察酶解温度、酶解时间、酶添加量和酶解p H 4个因素对多糖提取率的影响并进行验证试验。结果:果胶酶法提取棉花根多糖的最优工艺为酶解温度40℃、酶解时间150 min、酶添加量2.0%、酶解p H4.6。在此条件下,棉花根多糖的平均提取率为2.474%(RSD=3.34%,n=5)。结论:果胶酶法是一种有效的棉花根多糖提取方法;本试验优选工艺合理、可行。
OBJECTIVE：To optimize the extraction technology of polysaccharides from Gossypium herbaceum L.by pectinase hydrolysis.METHODS：Polysaccharides were extracted from G.herbaceum L.by pectinase hydrolysis combined with traditional hot water extraction.With the extraction rate of polysaccharides as the evaluated index,single factor test and orthogonal design were applied to investigate the effects of 4 factors including enzymolysis temperature,enzymolysis time,the amount of enzyme and p H value on polysaccharides extraction rate,and verification tests were conducted.RESULTS：The optimal extraction technology was as follows as enzymolysis temperature of 40 ℃,enzymolysis time of 150 min,enzyme accounting for 2.0%,p H value for enzymolysis of 4.6.Under the above conditions,the average extraction rate of polysaccharides from G.herbaceum L.was 2.474%（RSD=3.34%,n=5）.CONCLUSIONS：Pectinase hydrolysis is an effective method to extract polysaccharides from G.herbaceum L..The optimal extraction technology is reasonable and feasible.
出处
《中国药房》
CAS
北大核心
2015年第22期3131-3133,共3页
China Pharmacy
基金
安徽省高等学校省级优秀青年人才基金项目(No.2012SQRL126)
2013年地方高校国家级大学生创新创业训练计划项目(No.201310368030)
关键词
果胶酶
棉花根
多糖
提取
单因素试验
正交设计
Pectinase
Gossypium herbaceum L.
Polysaccharides
Extraction
Single factor test
Orthogonal design
