摘要
目的：研究菌毒清颗粒体外抗病毒的药效学。方法：将柯萨奇病毒B族3型（Cox B3）Nancy株、呼吸道合胞病毒（RSV）分别接种于人喉癌上皮Hep2细胞以复制细胞病毒模型。菌毒清颗粒原液（2 mg/ml）与氯原酸原液（2 mg/ml）以1∶2~1∶256倍比稀释后作用于病毒模型细胞,以Reed-Muench法计算50%细胞毒性浓度（TC50）和最大无毒浓度（TC0）。以1.25、0.625、0.312 5、0.15625 mg/ml菌毒清颗粒溶液与0.5、0.25、0.125、0.062 5 mg/ml绿原酸溶液培养病毒模型细胞,显微镜下评价细胞病变程度。以1.25、0.625、0.312 5、0.156 25、0.078 125 mg/ml菌毒清颗粒溶液与0.5、0.25、0.125、0.062 5、0.031 25 mg/ml绿原酸溶液作用于病毒模型细胞,Reed-Muench法计算对RSV、Cox B3细胞的50%抑制浓度（IC50）和治疗指数（TI）。结果：菌毒清颗粒TC50、TC0分别为1.79、1.25mg/ml,绿原酸TC50、TC0分别为0.71、0.5 mg/ml。当菌毒清颗粒溶液质量浓度为1.25 mg/ml时病毒感染细胞生长正常。菌毒清颗粒IC50均为0.22 mg/ml,TI均为8.14;绿原酸IC50分别为0.18、0.36 mg/ml,TI分别为3.94、1.97。结论：菌毒清颗粒具有体外抗Cox B3和RSV病毒的作用。
OBJECTIVE：To study in vitro antiviral effect of Junduqing granules.METHODS：Human laryngeal cancer Hep2 cells were inoculated with the Nancy strain of coxsackievirus B3（Cox B3） and respiratory syncytial virus（RSV） to establish virus-infected cell models.2 mg/ml original liquid of Junduqing granules and 2 mg/ml original liquid of chlorogenic acid which were diluted according to the ratio of 1 ∶ 2-1 ∶ 256 were used to act on the virus-infected cells.Reed-Muench method was adopted to calculate 50% toxic concentration（TC50）and maximal atoxic concentration（TC0）.Virus-infected cells were cultured with solution of Junduqing granules of 1.25,0.625,0.312 5 and 0.156 25 mg/ml and chlorogenic acid solution 0.5,0.25,0.125,0.062 5 mg/ml,and then the degree of cytopathic effect was evaluated with a microscope.Virus-infected cells were cultured with 1.25,0.625,0.312 5,0.156 25,0.078 125 mg/ml solution of Junduqing granules and 0.5,0.25,0.125,0.062 5,0.031 25 mg/ml of chlorogenic acid solution,and Reed-Muench method was adopted to calculate 50% inhibitory concentration（IC50）and therapeutic index（TI）on RSV and Cox B3 cells.RESULTS：TC50of Junduqing granules was 1.79 mg/ml and TC0 was 1.25 mg/ml,TC50 of chlorogenic acid was 0.71 mg/ml and TC0 was 0.5 mg/ml.Virus-infected cells grew normally when the mass concentration of solution of Junduqing granules was 1.25 mg/ml.IC50 of Junduqing granules was 0.22 mg/ml and TI was 8.14;IC50of chlorogenic acid were 0.18 and0.36 mg/ml,TI were 3.94 and 1.97.CONCLUSIONS：Junduqing granules have in vitro anti-Cox B3 and RSV effect.
出处
《中国药房》
CAS
北大核心
2015年第22期3070-3071,共2页
China Pharmacy
基金
广东省中医药强省科研课题(No.2010337)
