摘要
研制了一种新型微电流放大器系统,用于检测α-Hemolysin生物蛋白纳米通道在单分子检测实验中所产生的微弱电流信号(<100 p A)。在1 mol/L KCl、10 mmol/L Tris-HCl,1 mmol/L EDTA的缓冲液(p H 8.0)中测定了DNA-PEG-DNA交联物与纳米通道的穿越和碰撞信号。实验中使用3 k Hz贝塞尔滤波器和100 k Hz模数转换器来对电流进行采样。结果表明,此放大器系统能够有效降低电流记录过程中的噪音,有利于分辨待测物分子与纳米通道作用所产生的较小阻断的电流信号(<10 p A)。
A novel amplifier system was proposed for low-noise recording of pico-ampere current in nanopore experiment （〈100 pA）. As an example, the amplifier system was applied in α-hemolysin based nanopore detection of DNA-PEG-DNA conjugate to record the signals of translocation and bumping events in buffer solution （1 mol/L KCl, 10 mmol/L Tris--HCl, 1 mmol/L EDTA and pH=8. 0）. The amplified current signal was filtered by a 3 kHz Bessel filter and sampled by a 100 kHz analog-digital convertor. As a result, the presented amplifier system could lower the noise in recording the current. The current blockages （〈10 pA） of single molecules with low amplitude were recovered due to the high signal-to-noise ratio.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2015年第7期971-976,共6页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金委(Nos.21125522
21327807
51407078)
中央高校基本科研业务费专项资金(Nos.222201313004
WH1514049)资助项目~~
关键词
纳米通道
单分子检测
电流放大器
降噪
Nanopore
Single molecule detection
Current amplifier
Denoising
