摘要
目的分析不育男性精子碱性核蛋白,评价其作为男性不育症临床诊断指标的可行性。方法提取精子总碱性核蛋白(TNBP),电泳扫描测定各蛋白条带相对含量,用总组蛋白/总精蛋白(TH/TP)及人精蛋白1/人精蛋白2+人精蛋白3(HP1/HP2+HP3)比值评价精子碱性核蛋白相对含量。结果在1050例不育患者中,异常比例达81.05%,其中TH/TP异常53例(占5.05%);HP1/HP2+HP3异常73例(占6.95%);两项指标均异常725例(占69.05%)。结论精子碱性核蛋白有可能作为临床评价精子生育力的重要指标。
Objective: To analyze the sperm nuclear basic protein of the infertile men and evaluate the possibility of it as a clinical parameter for male infertility.Methods: Total nuclear basic protein (TNBP) was extracted from sperms of infertile men. Polyacrylamide gel electrophoresis was used to separate the TNBP and the individual proteins were quantified by scanning microdensitometry. TH (total histones)/TP (total protamines) and human protamine (HP) 1/HP2+HP3 ratios were used to represent the relative amount of sperm nuclear basic proteins.Results: The results indicated that 81.05% of total cases had aberrant sperm nuclear basic proteins. The ratios of TH/TP and HP1/HP2+HP3 were altered in 53 cases (5.05%) and 73 cases (6.95%) respectively. Two parameters were both altered in 725 cases (69.05%).Conclusion: The sperm nuclear basic protein could be an important parameter in the assessment of male fertility.
出处
《生殖医学杂志》
CAS
2005年第3期135-138,共4页
Journal of Reproductive Medicine
基金
国家攀登计划(970211019)
关键词
不育症
精子碱性核蛋白
精蛋白
Infertility
Sperm nuclear basic protein
Protamine
