摘要
目的本实验采用Real-time PCR的方法分别检测了VDR在72例乳腺疾病中RNA水平,探讨VDR是否为乳腺癌疾病进展过程中的一个作用点,并能否作为治疗乳腺癌的新的分子靶点提供一定的依据。方法本实验用Real-time PCR的方法检测VDR在18例腺病、18例原位癌、18例浸润性导管癌及其他类型癌(大汗腺癌、粘液癌、髓样癌、腺样囊腺癌、小叶癌、导管内乳头状癌各3例)中RNA水平的变化。结果腺病组VDR的RNA水平高于癌(浸润性导管癌及其他类型癌,P<0.05),浸润性导管癌组VDR的RNA水平较其他癌(原位癌、大汗腺癌、粘液癌、髓样癌、腺样囊腺癌、小叶癌、导管内乳头状癌)低差异有统计学意义(P<0.05)。结论 VDR在不同种类的乳腺癌发展过程中起着不尽相同的作用,且有可能是乳腺癌疾病进展过程中的一个作用点,并且作为治疗乳腺癌可能的新分子靶点提供了一定的依据。
Objective This article explored the RNA level of VDR in different mammary gland diseases,laying a solid foundation for the theory that VDR would play a key role in the progression of breast cancer and act as a new molecular target of breast cancer treatment. Methods We used real-time PCR method to detect changes of the vitamin D receptor RNA level in breast tissues including 18 cases of adenosis,ductal carcinoma in situ,invasive ductal carcinoma and 3 cases of apocrine carcinoma,mucinous carcinoma,medullary carcinoma,adenoid cystic carcinoma,lobular carcinoma,intraductal papillary carcinoma,respectively.Results The level of VDR RNA expression in the adenosis group is higher than that in invasive ductal carcinoma and other types of breast cancer（ P〈0. 05）. The level of VDR RNA expression in invasive ductal carcinoma is lower than that in other types of breast cancer（ P〈0. 05）. Conclusion VDR has various functions in the development of different types of breast cancer,indicating that VDR would play a key role in the progression of breast cancer and act as a new molecular target of breast cancer treatment.
出处
《四川医学》
CAS
2015年第7期918-919,共2页
Sichuan Medical Journal
基金
四川省卫生厅科研项目(编号:110031)
