摘要
目的探讨3种方法检测C-反应蛋白(CRP)对小儿长期发热的临床意义。方法采用免疫荧光干式定量法、散射比浊法、透射比浊法检测CRP的含量。结果实验组免疫荧光干式定量法检测CRP含量较对照组显著增高,差异具有统计学意义(P<0.05);散射比浊法及透射比浊法检测CRP含量较对照组均显著增高,差异均具有统计学意义(P<0.01)。与散射比浊法及透射比浊法比较,免疫荧光干式定量法检测CRP含量显著增高;而与散射比浊法比较,透射比浊法检测CRP含量显著降低。结论 3种检测方法中,免疫荧光干式定量法最简便,速度最快。散射比浊法最灵敏,准确度最高。透射比浊法较灵敏,准确度较免疫荧光干式定量法高,但较散射比浊法低。
Objective To investigate the clinical significance of three methods for the test of C - reactive protein（CRP） in children with long -term fever. Methods Dry type quantitative immunofluorescence method, Nephelometry method and Turbidi- metric method were used to detect the content of C - reactive protein. Results The content of CRP in the experimental group was significantly higher than that in the control group, the difference was statistically significant （P 〈 0.05 ） ; the result of C - reactive protein tested in experimental group was higher than that in control group by the scattering turbidity method and transmission turbidity method, and the difference was statistically significant（ P 〈 0.01 ）. Compared with the scattering and transmission turbidity, the content of CRP was significantly higher than that in the dry method, and the CRP content was significantly lower than that in the turbid method. Conclusion Among the 3 test methods, dry type quantitative immunofluorescence method is the most fast and simple method. Scattering turbidity method is the most sensitive and accurate method. Turbidimetric method is more sensitive and the accuracy is higher than the dry type quantitative immunofluorescence method but lower than the scattering turbidity method.
出处
《中国卫生检验杂志》
CAS
2015年第13期2143-2144,共2页
Chinese Journal of Health Laboratory Technology
关键词
免疫荧光干式定量法
散射比浊法
透射比浊法
小儿发热
C-反应蛋白
Dry type quantitative immunofluorescence method
Scattering turbidity method
Turbidimetric method
Fever in children
C - reactive protein
