摘要
目的研究新疆地区汉族房颤患者GGCX基因rs11676382位点多态性及其与华法林稳定剂量的相关性。方法从长居新疆地区的汉族房颤患者中，收集200例获得华法林稳定剂量的病例资料，所有入选者均采集外周血提取基因组DNA，采用聚合酶链反应，限制性片段长度多态性技术（PCR-RF凹）检查患者GGCX基因您11676382多态性的分布。比较不同基因型受试者之间华法林用量的差异。结果200例受试者中，GGCX基因阳11676382位点CC、CG和GG基因型的例数分别为150例（82．5％）、48例（16．5％）和2例（1．0％）；C和G等位基因的频率分别为99％、1％；所选人群GGCX（rs11676382）等位基因频率和基因型频率均符合HaZy—Weinberg平衡（x^2=0．744，P=0．388），本组受试者稳定剂量范围是1．25，6．88mg／d，平均（3．22±1．12）mg／d。基因型为CC、CG和GG的受试者华法林稳定剂量分别为（5．93±1．33）mg／d、（3．22±0．88）mg／d和（3．16±1．40）mg／d。经秩和检验分析，GGCX（M11676382）多态位点与华法林稳定剂量显著相关（x^2=6．094，P=0．047）。结论获得了长居新疆地区汉族房颤患者的GGCX基因巧11676382多态性。同时发现基因型CC与高剂量相关，GG与低剂量相关，该位点可作为预测个体华法林稳定剂量的一项指标。
Objective In order to study if The Correlation between GGCX gene polymorphism and the relationship on warfarin stable dosage in Xinjiang han nationality with atrial fibrillation （Atrial Fibrillation AF） . Methods The blood was collected from 200 han nationality people who live in Xinjiang for long time and have atrial fibrillation, DNA was extracted, polymerase chain reac- tion-restriction enzyme fragment length polymorphism （PCR-RFLP） was used and identified by agarose eletrophoresis to analyse GGCX genotype and allele distribution. Results The result showed that GGCX gene CC, CG and GG gene type frequency we-re found respectively 82. 5 % , 16. 5 % and 1 % , and C and G allele frequency were 99 % , 1%. The GGCX （rs11676382） allele and genotype frequencies of selected crowd were the I-Iardy-Weinberg balance （ x^2=0. 744, P = 0. 388 ） . The subject on a stable dose range was 1. 25 -6. 88 mg/d, mean 3.22 ± 1.12 rag/d, The genotype of themwere CC： 5.93 ±1.33 mg/d, CG： 3.22 ±0.88 mg/d and GG： 3.16 ±1.14 mg/d. The Kruskal-Wallis test analysis ： GGCX （rs11676382） polymorphisms were significantly associated with warfarin stable dose （ x^2 = 6. 094, P = 0. 047 ） . Conclusion Obtained GGCX gene rs11676382 polymorphism of xinjiang han patients with atrial fibrillation. The authors consider that warfarin high doses associated with genotype GGCX and low-dose-related. This can be used as an indicator to pre- dict an individual stable doses of warfarin.
出处
《医学分子生物学杂志》
CAS
2015年第4期202-206,共5页
Journal of Medical Molecular Biology
基金
基金项目：国家自然科学基金（No.81060017）
关键词
新疆
GGCX
汉族
心房纤颤
华法林
xinjiang
GGCX
Han nationality
atrial fibrillatin
warfarin
