摘要
基于牦牛胰脏提取胰酶,以酶活力和收率作为衡量指标,在单因素实验的基础上通过正交实验,确定牦牛胰酶制备的最佳工艺,最后探讨胰酶水解酪蛋白制备水解乳蛋白的效果。结果表明,牦牛胰酶最佳制备工艺为牦牛胰脏∶25%乙醇(提取剂)=1∶1,提取激活时间为5h,激活p H=6.8,75%乙醇沉淀8h。所得胰酶中胰蛋白酶、胰淀粉酶和胰脂肪酶的酶活力分别为1867、16298和5683 U/g,收率达9.7%;用其在温度40℃,p H7.5,酶与底物比例为1∶5的条件下水解酪蛋白4 h后所得的水解乳蛋白与Hyclone的水解乳蛋白相比较,有19种相同的氨基酸,且氨基酸总含量高于Hyclone的水解乳蛋白,分别为36.111%和27.725%。综上可知,该工艺简单,耗时短,易于产业化,在水解蛋白质领域中具有良好的应用前景。
Pancreatin was extracted from yak pancreas, enzyme activity and yield was used as the measurement index.On the basis of single factor experiment, the orthogonal test was used to determine the optimization of technological parameters of yak pancreatin preparation, at the same time,the effects of hydrolyzing casein was investigated using pancreatin to prepare lactoalbumin hydrolysate. The results showed that the optimum technological condition for preparation of yak pancreatin was as follows： yak pancreas：25% ethanol （extracting agent） = 1 ： 1, extraction and activation time was 5 h, pH = 6.8,75% ethanol precipitated for 8 h. Under these conditions,enzyme activity of trypsin, amylase and lipase were 1867,16298 U/g and 5683 U/g respectively, the yield reached 9.7%.Then the pancreatin add to casein with ratio of 1 ：5 to prepare lactoalbumin hydrolysate under 40 ℃,pHT.5 for 4 h. Consistant with Hyclone,there were 19 kinds of amino acids in hydrolysis product, but the contants of total amino acids were higher than Hyclone ：the percentage was 36.111% and 22.725% respectively.In conclusion,this technique is simple, time saving, feasibility to be industrialized, and will be a good application prospect in the field of protein hydrolysate.
出处
《食品工业科技》
CAS
CSCD
北大核心
2015年第15期202-207,共6页
Science and Technology of Food Industry
基金
教育部"长江学者和创新团队发展计划"项目(IRT13091)
兰州市科技计划项目(2014-1-158)
国家民委创新团队计划资助([2013]231号)
国家外传项目(GDW20146200235)
关键词
牦牛胰脏
胰酶
酶活力
收率
水解乳蛋白
yak pancreas
pancreatin
enzymatic activity
yield
lactoalbumin hydrolysate
