摘要
采用不同培养液、不同培养温度不同贮藏时间和温度、-80℃超低温贮藏花粉不同解冻方法对马银花(Rhododendron ovatum)的花粉萌发特性及贮藏特性进行研究,结果表明:在25℃下,用100 g/L蔗糖+100 mg/L硼酸+100 mg/L GA3的离体培养液,培养4h花粉萌发率高,可达80%,GA3能促进花粉萌发和花粉管生长;各贮藏温度下的花粉贮藏15 d萌发率都保持在高水平,但室温贮藏花粉45 d失活,3.3℃贮藏花粉45 d萌发率可保持在36.11%,可作为生产应用的贮藏温度;-80℃贮藏的花粉采用30℃水浴解冻2 min效果较好。
Experiments were conducted on characteristics of pollen germination and storage of Rhododendron ovatum with different culture solution and temperature, storage duration and temperature, thawing methods after storage under-80℃. The results showed that pollen germination rate topped to 80% with culture medium of 100 g/L sucrose＋100 mg/L boric acid ＋100 mg/L GA3 at 25℃ for 4 hours. The experiments indicated that pollen germination rate and growth of pollen tubes were promoted significantly by GA3. The germination rate kept high 15 days later under each tested storage temperature. The pollen was inactivation 45 days after storage at room temperature. But the pollen germination rate kept 36.11% after storage at 3.3℃ for 45 days. Pollen（stored at-80℃） thawed with 30℃ aqueous bath for 2 minutes had better effect of germination rate.
出处
《浙江林业科技》
北大核心
2015年第1期49-53,共5页
Journal of Zhejiang Forestry Science and Technology
基金
浙江省省院合作林业科技项目(2012SY02-1)
关键词
马银花
花粉
萌发
贮藏
Rhododendron ovatum
pollen
germination
storage
