摘要
目的：观察苦楝树皮提取物中不同极性组段对斜纹夜蛾幼虫的毒杀与拒食作用,寻找活性成分主要存在的组段。方法：苦楝树皮经95%乙醇冷浸提取,浓缩成浸膏,用乙酸乙酯萃取后得到粗提物,运用硅胶柱层析分离为不同极性的15个组段（K1～K15）,采用叶碟法测定不同极性组段在低剂量（10-6 g/m L）、中剂量（10-4 g/m L）、高剂量（10-2 g/m L）下对斜纹夜蛾幼虫的取食叶面积与死亡率的影响。结果：苦楝树皮提取物对斜纹夜蛾幼虫具有明显的拒食作用,且与剂量成正比。而K5、K6、K10组段在10-2 g/m L浓度下表现出了强烈的毒杀作用,死亡率为100%。K11组段对斜纹夜蛾幼虫也有一定的毒杀作用,死亡率为33.3%。K5、K6组段在10-6 g/m L浓度下,均有较好的拒食作用。结论：K5、K6组段比K10、K11组段对斜纹夜蛾幼虫具有更显著的拒食与毒杀作用,且在K5、K6组段中有望分离得到单一活性成分。
Objective：In order to find new compounds with strong antifeeding activities,the crude extract and fractions from barks of Melia azedarach L.were selected for their antifeeding and toxic activity against Spodoptera litura larvae.Methods：The 95% ethanol extract of the barks of Melia azedarach L.was concentrated,and the aqueous solution was partitioned with ethyl acetate.The ethyl acetate extract was subjected to silica gel CC eluted with ether-ethyl acetate to give 15 fractions（K1～K15）.The fractions were investigated against Spodoptera litura larvae by leaf disc method to test the leaf area and the mortality.Results：Ethyl acetate extract from barks of Melia showed significant antifeeding activity against Spodoptera litura larvae,which was improved with increased dosages.Fractions 5,6,10 displayed strong toxic effects,and Fraction 11 also indicated toxic effects.Fractions 5,6 revealed strong antifeeding activity even at the low concentration.Conclusion：Fractions 5,6 demonstrate obvious antifeeding and toxic activity against Spodoptera litura larvae than Fractions 10,11.The fractions which reflect stronger antifeeding activity will be isolated and purifies in hoping of achieving the new active compounds.
出处
《温州医学院学报》
CAS
2015年第8期552-555,共4页
Journal of Wenzhou Medical College
基金
浙江省自然科学基金资助项目(LY12B02007)
关键词
苦楝树皮
乙酸乙酯提取物
斜纹夜蛾
拒食活性
毒杀作用
Melia azedarach barks
ethyl acetate extract
Spodoptera litura larvae
antifeedant
toxic
