摘要
以扬麦16和徐麦30为试验材料,利用人工气候室模拟低温逆境,研究拔节期-3℃和-5℃低温胁迫对小麦植株受冻率、叶片内源激素含量和抗氧化酶活性的影响.结果表明:随着处理温度的降低、胁迫时间的延长,小麦植株冻害等级与冻害指数增加,-5℃处理72 h两品种五级冻害率均为100%.低温处理结束当天,小麦叶片中内源激素脱落酸(ABA)、玉米素核苷(ZR)含量、抗氧化酶超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性随胁迫程度加重呈先升高后降低的趋势;处理结束后3 d,ABA、ZR含量及抗氧化酶活性较处理结束当天升高;至处理结束后6 d,与自然生长的对照处理接近.低温胁迫叶片中赤霉素(GA3)含量下降,处理结束后3和6 d,扬麦16叶片中GA3含量呈上升趋势,徐麦30则表现为先升高后下降.-5℃72 h重度胁迫处理叶片中ABA、ZR、GA3含量和SOD、POD、CAT活性均较对照显著下降.相关分析表明,较高的ABA、ZR含量、SOD、POD、CAT活性以及较低的GA3含量可减缓低温胁迫对小麦植株的伤害.
Low temperature stresses （ -3 and -5 ℃ ） were simulated using artificial temperature- controlled phytotrons to study the freezing rate, the contents of endogenous hormones, and the acti- vities of antioxidative enzymes in the leaves of wheat plants of Yangmai 16 （YM 16） and Xumai 30 （ XM 30） at jointing stage. The grade and index of freezing injury increased with lower temperature and longer stress. The freezing rate was at the 5th level and the main stems and tillers of both culti- vars were finally dead under -5 ℃ lasting for 72 h. On the last day of stress initiation, the contents of abscisic acid （ABA） and zeatin riboside （ZR）, and the activities of superoxide dismutase （SOD） , peroxide dismutase （POD） , and catalase （CAT） in leaves increased at the beginning and then declined as low temperature progressed. On the 3rd day after stress, the contents of ABA and ZR and the activities of antioxidative enzymes were higher than those on the last day of cold stress, and then reduced to the level of the control on the 6th day after stress. The content of gibberellins （GA3） was lowered by cold stress. For YM 16, GA3 content increased from the 3rd day to the 6th day after cold stress, whereas, for XM 30, it increased first and then decreased. For the treatment of -5 ~C lasting for 72 h, the contents of hormones and the activities of antioxidative enzymes were significantly lower than those of the other treatments. Correlation analyses showed that higher ABA and ZR contents, and higher SOD, POD and CAT activities as well as lower GA3 content could al- leviate the low-temperature injury in wheat plants under low temperature stress.
出处
《应用生态学报》
CAS
CSCD
北大核心
2015年第7期2015-2022,共8页
Chinese Journal of Applied Ecology
基金
国家自然科学基金项目(31000683)
扬州大学科技创新培育基金项目(2014CXJ037)
江苏省科技支撑计划项目(BE2012467)
江苏高校优势学科建设工程项目资助
关键词
小麦
低温
内源激素
抗氧化酶
wheat
low temperature
hormone
antioxidative enzymes.
