摘要
研究了低温(0￣5℃)胁迫下钼对冬小麦抗氧化系统和膜脂过氧化的影响。结果表明,低温胁迫下施钼植株电解质渗漏率和丙二醛含量显著降低。施用钼肥提高了冬小麦叶片中抗氧化酶类如超氧化物歧化酶(SOD,EC1.15.1.1)、过氧化物酶(POX,EC1.11.1.7)和过氧化氢酶(CAT,EC1.11.1.6)的活性。低温胁迫下施钼对抗氧化酶(SOD、POX 和 CAT)活性提高幅度比常温下高。不论常温还是低温下,施钼均提高了冬小麦叶片中抗坏血酸和脯氨酸含量,低温胁迫下提高幅度更大。常温下缺钼和施钼处理后,叶片中类胡萝卜素含量差异不显著;低温下施钼后,冬小麦叶片类胡萝卜素含量显著增加。因此,低温胁迫下施钼植株活性氧清除能力增强、细胞膜伤害减轻可能是冬小麦抗寒力提高的原因之一。
Effects of molybdenum （Mo） on antioxidative defense system and membrane lipid peroxidation in leaves of winter wheat （Triticum aestivum H. vat. ‘Huamai 8＇） were investigated under low temperature stress. Results of experiments indicate that Mo application in winter wheat induced a dramatic decrease in electrolyte leakage and malondialdehyde content under low temperature stress. The activities of antioxidative enzymes such as superoxide dismutase （SOD, EC 1.15.1.1）, peroxidase （POX, EC 1.11.1.7） and catalase （CAT, EC 1.11.1.6） were increased by Mo application and the extents of increase at low temperature were higher than those at normal temperature. Mo application also caused a significant increase in the ascorbic acid （AsA） and proline contents both at normal and low temperature and following the low temperature stress the increases in ascorbic acid and proline contents in Mo-treated winter wheat were higher. There was no significant difference in carotenoid （CAR） content between with and without Mo treatment under normal temperature, while there was a significant increase in Mo treatment under low temperature stress. It could be speculated that Mo application enhanced cold resistance by increasing the capacity to scavenge active oxygen species and alleviating membrane damage in winter wheat under low temperature stress.
出处
《植物生理与分子生物学学报》
CAS
CSCD
北大核心
2006年第2期175-182,共8页
Journal Of Plant Physiology and Molecular Biology
基金
教育部博士点基金项目
教育部"新世纪优秀人才支持计划"项目(No.NCET-04-0731)资助。~~
关键词
钼
冬小麦
活性氧
抗氧化酶
膜脂过氧化
molybdenum
winter wheat
reactive oxygen species
antioxidative enzymes
membrane lipid peroxidation
