摘要
为获得MsMYB2基因过量表达的转基因拟南芥(Arabidopsis thaliana)植株,利用PCR技术在紫花苜蓿(Medicago sativa)中克隆出MYB2基因并命名为MsMYB2,MsMYB2基因编码区全长834 bp,编码1条长度为278个氨基酸的多肽链。通过DNA重组技术将其与pBI121连接,成功构建了植物表达载体pBI-MsMYB2。通过花序侵染法获得具有卡那霉素抗性的转基因拟南芥植株,并通过PCR和RT-PCR对目的基因进行检测,结果证明目的基因已整合到拟南芥基因组中并且可以表达,成功获得了MsMYB2基因表达的拟南芥转基因植株。
A gene named MsMYB2 was cloned from alfalfa by PCR technique with 834 bp coding domain sequence encoding 278 amino acids. The MsMYB2 gene was inserted into plasmid pBI121 by DNA recombination technology; and a plant expressing vector pBI-MsMYB2 was constructed. The plants of transgenic Arabidopsis thaliana with kanamycin resistance were screened through floral dip method and analyzed by PCR and RT-PCR. The results showed that the target gene was inserted into the genomes of Arabidopsis and expressed. Therefore, the transgenic Arabidopsis thaliana plants expressing MsMYB2 gene were successfully obtained in this study.
出处
《草地学报》
CAS
CSCD
北大核心
2015年第3期580-585,共6页
Acta Agrestia Sinica
基金
国家牧草产业技术体系(CARS-35)专项
国家"十二五"科技支撑计划子课题"豆科及禾本科牧草良种扩繁技术研究与示范"专项(2011BAD17B01-02)资助
关键词
紫花苜蓿
MsMYB2基因
拟南芥
转化
Medicago sativa L.
Arabidopsis thaliana
MsMYB2 gene
Transformation
