摘要
目的探讨BMSCs对乙酸胃溃疡愈合质量的影响和机制。方法取清洁级雄性Wistar大鼠48只,用乙酸诱导大鼠胃溃疡模型,造模后第3天随机分为3组,每组16只。A组仅打开腹腔,拉出胃;B、C组打开腹腔拉出胃后,在溃疡周围胃壁浆膜下5个不同点分别注入150μL PBS和150μL第4代BMSCs PBS液(细胞密度为1×108个/100μL)。第10天测量溃疡面积,组织学方法检测再生胃黏膜厚度和扩张腺体数,免疫组织化学染色方法检测溃疡边缘VEGF的表达。结果 C组溃疡面积显著低于A、B组(P<0.01);A、B组间比较差异无统计学意义(P>0.05)。HE染色示,C组溃疡边缘再生胃黏膜较A、B组厚,扩张的腺体较少,黏膜结构较规则。C组再生胃黏膜厚度显著厚于A、B组(P<0.01),扩张腺体数显著少于A、B组(P<0.01);A、B组间比较差异均无统计学意义(P>0.05)。免疫组织化学染色示,C组溃疡边缘胃黏膜VEGF阳性表达显著多于A、B组。C组VEGF表达积分吸光度(IA)值显著高于A、B组(P<0.01);A、B组间比较差异无统计学意义(P>0.05)。结论 BMSCs通过分泌VEGF加速胃溃疡愈合,提高溃疡愈合质量。
Objective To explore the effect and mechanisms of bone marrow mesenchymal stem cells（BMSCs）on healing quality of acetic acid-induced gastric ulcer. Methods Forty-eight clean grade male Wistar rats were used to establish the model of gastric ulcer with acetic acid and were randomly divided into 3 groups after 3 days of modeling,16 rats each group. After the abdominal cavity was open and stomach was pulled out, no treatment was given in group A, 150 μL phosphate buffered saline（PBS） and 150 μL BMSCs at passage 4＋PBS（1×108 cells /100 μL） were injected into the gastric wall surrounding the ulcer at 5 different points in groups B and C respectively. After 10 days, the ulcer area was measured, the mucosal thickness and the number of dilated glands were tested in the regenerative mucosa by histological method. And the expression of vascular endothelial growth factor（VEGF） was detected at ulcerative margin by immunohistochemical method. Results The ulcer area in group C was significantly smaller than that of groups A and B（P〈0.01）, but no significant difference was found between groups A and B（P〉0.05）. HE staining showed that group C had thicker regenerative gastric mucosa, less dilated glands, and more regular mucosal structure than groups A and B, showing significant differences in regenerative gastric mucosa thickness and dilated glands number（P〈0.01）,but no significant difference between groups A and B（P〉0.05）. Immunohistochemical staining showed that the positive expression of VEGF in the ulcer margin mucosa of group C was significantly higher than that of groups A and B. The integral absorbance（IA） value of VEGF expression in group C was significantly higher than that in groups A and B（P〈0.01）, but no significant difference between groups A and B（P〉0.05）. Conclusion BMSCs can accelerate ulcer healing by the secretion of VEGF, and improve the quality of ulcer healing.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2015年第7期889-892,共4页
Chinese Journal of Reparative and Reconstructive Surgery
基金
黑龙江省教育厅科学技术研究项目(12531803)~~
关键词
BMSCS
胃溃疡
VEGF
溃疡愈合
大鼠
Bone marrow mesenchymal stem cells
Gastric ulcer
Vascular endothelial growth factor
Ulcer healing
Rat
