摘要
为了比较广西猪种和国外3个猪种的遗传资源多样性和进化关系,利用文献报道的1对引物测定了广西5个地方猪种(巴马香猪、东山猪、桂中花猪、陆川猪、环江香猪)共85个样本的mtDNA D-loop序列,结合已报道的国外3个猪种(杜洛克猪、大约克猪和长白猪)的45条mtDNA D-loop序列,进行单倍型、遗传多样性和系统发育树分析。结果表明,8个群体样本的130条序列共定义38种单倍型;广西各猪种之间遗传距离较小,远小于其与国外猪种杜洛克猪、长白猪间的遗传距离;构建的NJ分子系统进化树将所分析样本分为2个独立的支系:一支包含了所有广西地方猪种和部分大约克猪的单倍型,另一支则全部是3个国外猪种的单倍型,广西猪种和3个国外猪种有着两个明显不同的母系起源。陆川猪在系统树上分布较为紧密,其他4个猪种都很分散,表明陆川猪母源血统单一,其他4个猪种母源血统遗传背景比较复杂,彼此之间有基因交流。广西猪种母系资源没有受到国外引进猪种资源的影响,在其开发利用中依然适合用作母本。
In order to compare the genetic diversity and phylogenetie relationships between Guan- gxi pig breeds and three exotic pig breeds, one pair of primers was used to amplify 85 mtDNA D-loop sequences of five Guangxi pig breeds （Bama Xiang pig, Dongshan pig, Guizhong spotted pig, Luchuan pig, Huanjiang Xiang pig）, and we downloaded 45 mtDNA D-loop sequences of three exotic pig breeds （Duroc,Yorkshire, Landrace） from GenBank. Then the haplotype,genetic diver- sity and phylogenetics analysis were carried out. The results showed that 38 haplotypes were iden- tified in 130 sequences,and the mean distances among Guangxi pig breeds were smaller than that among Duroc,Landrace and Guangxi pig breeds. The NJ phylogenetic tree classified all samples into two clusters. One contained all of Guangxi pig breeds and partial Yorkshire, the other was composed wholly of exotic pig breeds, which mean there were two distinct maternal origins. In the cluster of Guangxi pig breeds,Luchuan pig were clustered,and the others were rather dispersive. It indicated that Luehuan pig had simple maternal bloods,and the other breeds had complex ma- ternal bloods, and they had gene flow with each other. The maternal pig resources in Guangxi were not affected by the introduction of exotic Dig breeds,and they were still suitable for use as a female parent in their development and utilization.
出处
《中国畜牧兽医》
CAS
北大核心
2015年第7期1807-1815,共9页
China Animal Husbandry & Veterinary Medicine
基金
广西自然科学基金项目(2012GXNSFAA053071
2013GXNSFB019115)
