摘要
目的用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法测定医用涂敷系统体内降解主要产物聚乙二醇(PEG)15000的含量,评价其体内降解规律。方法将医用涂敷系统植入大鼠背部,分别在术后0,1,2,3,4,5,6,7,8,9,10,12周(n=6)大鼠脱颈处死,并用磷酸盐缓冲液冲洗,收集剩余凝胶及冲洗液,用HPLC-ELSD法测定剩余凝胶和冲洗液中聚乙二醇15000的含量。结果凝胶在大鼠体内至第3周末前完好,至第6周呈无色透明碎块,后向液态转化,至第9周呈现为水样,至第12周肉眼未见。剩余凝胶中聚乙二醇15000的含量随实验周数的延长而递减。结论聚乙二醇15000在大鼠体内降解速率呈现为慢—快—慢的规律,符合硬脑膜缺损修复生理过程的要求。
Objective To determine the content of polyethylene glycol15000（PEG15000）,the main degradation product of medical coating system inside the body by using high performance liquid chromatography-evaporative light scattering detector（HPLC-ELSD） and to evaluate its degradation rule.Methods The medical coating system was implanted into the back of rats,and these rats were executed by cervical vertebra dislocation zero,one,two,three,four,five,six,seven,eight,nine,ten,twelve weeks（n = 6） after operation,respectively and washed with phosphate buffer to collect the rest gel and washing fluid,then HPLC-ELSD was used to determine the content of PEG15000 in the rest gel and washing fluid.Results Gel in rats was intact until the end of the third week,presented as colorless transparent pieces until the sixth week,and then transformed to liquid state,presented as water until the ninth week and not seen by naked-eye until the twelfth week.The content of PEG15000 in the rest gel was decreased progressively with the extension of experiment weeks.Conclusion The degradation rate of gel inside the body of rates presents the rule of one fast and one slow; which conforms to the requirements in physiological process of dural defects healing.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2015年第13期1265-1267,共3页
The Chinese Journal of Clinical Pharmacology
