摘要
目的观察厄贝沙坦对高糖诱导胰岛NIT-1细胞Caspase-3 mRNA表达及凋亡的影响。方法将对数生长期胰岛NIT-1细胞分为空白对照组(A组),高糖组(25 mmol/L)(B组),高糖加厄贝沙坦(0.1 mmol/L)组(C组),厄贝沙坦(0.1 mmol/L)培养24 h后加高糖培养组(D组),各组细胞分别培养48 h。通过Hochest 33342荧光染色观察各组细胞形态,反转录聚合酶链反应(RT-PCR)技术检测各组细胞Caspase-3 mRNA的表达。结果 Hochset 33342荧光染色结果:A组细胞呈均匀弥漫性蓝光着色,B组细胞呈不均匀强蓝光着色,可见细胞核浓缩和细胞碎片,C组和D组大部分细胞着色与A组细胞一致,少数呈不均匀强荧光蓝色减少。RT-PCR结果:B组Caspase-3 mRNA表达量高于A、C、D组(P<0.05),A、C、D组间比较,差异无统计学意义(P>0.05)。结论高糖可诱导胰岛NIT-1细胞凋亡;厄贝沙坦可降低Caspase-3 mRNA表达量,减少细胞凋亡,在体外,对高糖诱导胰岛NIT-1细胞可能具有一定的保护作用。
Objective To observe the effect of irbesartan on the Caspase-3 mRNA expression and apoptosis of high glucose-induced islet NIT-1 cell.Methods The islet NIT-1 cells in logarithmic growing phase were divided into blank control group（Group A）,high glucose group（25 mmol/L,Group B）,high glucose＋irbesartan group（0.1 mmol/L,Group C）,and Group D in which the cells were cultured with irbesartan（0.1 mmol/L） for 24 hours and then received high glucose culture.The cells in each group were cultured for 48 hours.Hochest 33342 fluorescent staining was used to observe the cell morphology in each group, while reverse transcription polymerase chain reaction （ RT-PCR） was used to determine the Caspase-3 mRNA expression of the cells in each group.Results The result of Hochset 33342 fluorescent staining showed that the cells in Group A appeared uniformly diffuse blue light,in Group B appeared unevenly high-intensity blue light with concentrated cell nucleus and cell debris,and most of the cells in Groups C and D appeared blue light as same as that in Group A,a few cells appeared unevenly high-intensity fluorescent blue reduction.The result of RT-PCR showed the expression level of Caspase-3 mRNA in Group B was higher than that in Group A,Group C and Group D,respectively（P〈0.05）,and there was no significant difference in the expression level of Caspase-3 mRNA among Group A,Group C and Group D（P〉0.05）.Conclusion High glucose can induce the apoptosis of islet NIT-1 cells.Irbesartan can decrease the expression level of Caspase-3 mRNA as well as reduce the apoptosis,and it has certain protective effect on high glucose-induced islet NIT-1 cell in vitro.
出处
《广西医学》
CAS
2015年第5期603-605,共3页
Guangxi Medical Journal
基金
广西自然科学基金(2013GXNSFAA019202)
