摘要
目的探讨高浓度葡萄糖对人脐静脉血管内皮细胞系P38 MAPK和TGFβ2表达的影响,以进一步探讨糖尿病视网膜病变早期进展的机制。方法采用RT-PCR、Western blot法检测细胞P38 MAPK和TGFβ2表达变化。结果高浓度葡萄糖可使内皮细胞P38 MAPK和TGFβ2表达增加。结论高糖诱导糖尿病视网膜血管病变可能部分通过P38 MAPK和TGFβ2表达增强。
Objective To examine the effects of glucose on the expression of P38 MAPK and TGFβ2 in cultured human umbilical vein endothelial cells. Methods Human umbilical vein endothelial cells（HUVECs）were incubated in a culture medium with 11.2 mmol/L,16. 8 mmol/L and 33. 6 mmol/L glucose concentrations for 24 h,48 h and 72 h respectively. The expression P38 MAPK and TGFβ2 was measured by RT-PCR and Western-blot. Results When D-glucose concentrations rose, HUVECs exposed to high glucose concentration（ 11.2 mmol/L, 16. 8 mmol/L and 33.6 mmol/L） showd increase in cell expression of P38 MAPK and TGFI32 after 48 h and 72 h exposure as compared with those cultured in medium of low glucose concentration（5.6 mmol/L）. Conclusion High concentration of glucose can arrest the proliferative response. Even a short-term exposure of endothelial ceils （ECs）to high glucose concentration may lead to their activation associated with increased expression of P38 MAPK and TGFβ2. High Glucose Concentration on P38 MAPK and TGFβ2 expression may participate the pathogenesy of diabetic retinopathy.
出处
《基础医学与临床》
CSCD
北大核心
2007年第2期169-173,共5页
Basic and Clinical Medicine
