摘要
目的：研究缬草挥发性成分。方法：采用：以《药典（一部）》附录XD甲的标准方法提取缬草挥发性成分，采用GC－MS结合化学计量学方法对缬草挥发性成分进行定性分析。色谱柱：5 HP－MS；载气：He；流速：1．0mL／min；进样量：1μL；分流比：1∶100。质谱条件：电子轰击（ EI）离子源，辅助线温度280℃，离子源温度230℃，四级杆温度150℃；质量扫描范围：m／z 50～550。采用Chemstation和Nist 08谱库检索。结果：共鉴定出30种成分，占总含量的94％。结论：共鉴定出成分，与传统定性分析方法相比，GC－MS结合化学计量学方法具有较高的灵敏度和准确度，有效增加鉴定的成分，可用于缬草挥发性成分的定性分析。
Objective：To analyze the volatile components in Valeriana officinalis L.Methods： The volatile components were extracted by steam-stilling.GC-MS-AMDIS combined with Retention index technology was used for the quali -tative analysis of the volatile components in Valeriana officinalis L, 5HP-MS quartz capillary column （30m ＆#215;0.25mm ＆#215;0.25μm）; temperature programcarrier gas （ He） , velocity 1.0mL/min,the injection volume 1μL;split ratio 1∶100. MS conditions：electron impact （ EI） ion source, transmission line temperature 280 ℃, ion source temperature 230℃, Quadrupole temperature 150 ℃mass scan range m/z 50~550.And the results were contrasted with Chemstation and Nist 05a.Results：Totally, 30 compounds which accounted for 94%of the whole extracts of Valeriana officinalis L were identified.Conclusion：Compared with the traditional qualitative analysis method, GC-MS-AMDIS combined with re-tention index technology has a higher sensitivity and accuracy,and can be used for qualitative analysis of the volatile con-stituents of Valeriana officinalis L.
出处
《中医药学报》
CAS
2015年第3期17-19,共3页
Acta Chinese Medicine and Pharmacology
基金
国家自然科学基金面上项目(No.81173645)
黑龙江省实验动物科技发展专项资金(PC13S15)
黑龙江省自然科学基金面上项目(H201328)
黑龙江中医药大学校基金(2012-035082)
