摘要
目的:学习人正常黑素细胞的原代培养技术,为进一步研究提供实验模型。方法:分离培养人正常黑素细胞,细胞取材于包皮环切术后的包皮组织;采用左旋多巴染色法进行细胞鉴定。结果:成功培养、传代、纯化了人正常黑素细胞,并经左旋多巴染色证实。结论:动态观察了人正常黑素细胞培养的全过程,验证了细胞的特性,获得了大量纯化的黑素细胞,为进一步的细胞内干预实验提供了物质基础。
Objective： To study the methods of culturing human normal melanocytes,providing experimental models for further investigation.Methods： Cells from prepuce tissue after circumcision were isolated to culture normal human melanocytes which were later identified by Levodopa staining method. Result： Normal human melanocytes were cultured,reproduced and purified successfully,which was identified by Levodopa staining method. Conclusion： The whole culture process of normal human melanocytes has been dynamically observed. The characteristics of melanocytes have been identified. A large number of purified melanocytes have been obtained,which provides basic materials for further intervention.
出处
《包头医学院学报》
CAS
2015年第5期4-5,共2页
Journal of Baotou Medical College
关键词
黑素细胞
体外培养
原代培养
Human melanocytes
In vitro culture
Methods
