摘要
目的：建立海马神经细胞原代培养技术。方法：取新生1～3d的Wistar大鼠，开颅，迅速分离出海马组织在Hanks中剪碎，加入0．125％胰蛋白酶2mL消化，胎牛血清终止消化，细胞计数后种植于多聚赖氨酸包被的6孔培养板中。结果：神经细胞存活率高，纯度达90％以上，神经细胞生长良好。结论：用此方法能成功获取大鼠海马神经细胞。
Objective： To establish primary culture technique for rat hippocampal neuron. Methods： The neonatal Wistar rat （1-3d） were killed, the hippocampal tissue was separated and quickly broken into pieces in Hanks liquid, and then digested in 0. 125%trypsinase and ended to be digested with fetal cattle serum. The cells were counted and then grew in culture plate which was coated with polylysine. Results：The neuron livability was high and the pure degree reach 90% ,and the growth of neuron were good. Concl usion：Rat hippocampal neurons are obtained successfully by this way.
出处
《黑龙江医药科学》
2009年第4期20-21,共2页
Heilongjiang Medicine and Pharmacy
基金
黑龙江省教育厅科技项目资助(No.11523053)
