摘要
目的研究虫草菌丝对于改善5／6肾切除慢性肾衰大鼠肾组织纤维化的保护作用。方法按经典5／6（Ablation／Infarction，A／I）肾切除法制作慢性肾衰大鼠模型，随机分为模型组、虫草组，另设假手术组。给予相应干预，60d后检测大鼠血红蛋白、肾功能等生化指标，测定尾动脉血压，病理切片HE染色观察肾脏组织情况，Western blot法检测肾组织肿瘤坏死因子α（tumor necrosis factors-α，TNF-α）蛋白表达，实时定量PCR检测残余肾组织肾皮质和髓质中TNF-α mRNA表达。结果虫草组的血肌酐、血尿素氮较治疗前明显降低（P〈0．01），内生肌酐清除率明显升高（P〈0．01）。虫草组降低血肌酐、血尿素氮水平，升高内生肌酐清除率水平均优于模型组（P〈0．01）。与模型组比较，虫草组的血红蛋白值明显提高（P〈0．01），收缩压明显下降（P〈0．01），舒张压有所下降（P〈0．05）。肾组织病理显示，虫草组病理变化明显减轻，优于模型组。与假手术组比较，在模型组和虫草组皮质和髓质组织中TNF-α的蛋白表达、TNF-α mRNA表达均明显升高（P〈0．01）；与模型组比较，虫草组的皮质和髓质组织中TNF-α的蛋白表达TNF-α mRNA均明显减少（P〈0．01）。结论虫草菌丝能够改善慢性肾衰大鼠肾功能，减轻贫血，改善肾组织病理变化。这一作用机制可能与虫草菌丝抑制TNF-α生成、减轻慢性肾衰大鼠组织的微炎症状态有关。
Objective To investigate the effects of Caterpilar Fungus on renal fibrosis in the rats induced by 5 /6th kidney ablation/infarction. Methods Rats, which were made into chronic renal failure （CRF） animal models by means of 5/6 ablation/infarction, were divided randomly into model group （group B）, and Caterpilar Fungus group （group C） （n = 10 each）. Meanwhile, sham operation grourp （group A） was set up with 10 rats also. Each group was given relevant intervention treatment by administration for 60 successive days. Thereafter, SCr, BUN and Ccr were determined. The pathomorphologic changes of the nephridial tissue were observed. The expression of TNF-α mRNA in the remnant renal cortex and medulla was assessed by real-time PCR. Results （1） After intervention treatment, the levels of SCr and BUN were decreased （P〈0. 01）, and Ccr increased （P〈0. 01） in group C as compared with group B （P〈0. 01）. As compared with group B after treatment, HB was increased （P〈0. 01）, and SBP and DBP deseased in group C （P〈0. 01, P〈0. 05）. （2） The pathological changes of the nephridial tissue in group C were obviously ameliorated as compared with group β. （3） As compared with group A after treatment, the expression of TNF-α protein and mRNA （P〈0. 01） in the renal cortex and the medulla were obviously increased in groupd B and C, and that in group C was decreased as compared with group B （P〈0. 01 ）. Conclusions Caterpilar Fungus can improve renal functions, and alleviate anemia and pathological changes of nephridial tissues, which may be related to down-regulation of TNF-α and inhibition of micro-inflammatory state in renal tissues of rats with CRF.
出处
《临床肾脏病杂志》
2015年第5期302-305,共4页
Journal Of Clinical Nephrology
基金
国家自然科学基金项目(No.30973723)
教育部博士学科点专项基金项目(No.2012310711004)
上海市中医药事业发展三年行动计划(No.ZYSNXD-CC-MZY044)
关键词
肾纤维化
虫草菌丝
肿瘤坏死因子Α
Renal fibrosis
Caterpilar Fungus
Tumor necrosis factor-α
