摘要
目的检测子宫内膜异位症（EMS）患者血清和腹腔液中甲壳质酶蛋白（YKL）-40和CA125水平,并探讨YKL-40、CA125和EMS患者临床病理学特征间的关系及其相关性。方法选取2012年3月至2014年3月江苏省徐州市中心医院生殖医学中心收治的确诊为EMS的50例患者为研究对象,并纳入EMS组。选取同期在本院就诊并确诊为子宫良性肿瘤的40例患者纳入对照组。两组患者的年龄、体质指数、痛经所占比例、不孕所占比例及不孕时间比较,差异均无统计学意义（P〉0.05）。本研究遵循的程序符合江苏省徐州市中心医院人体试验委员会制定的伦理学标准,得到该委员会批准,分组征得受试对象的知情同意,并与之签署临床研究知情同意书。采用电化学发光法对患者血清和腹腔液CA125水平进行测定,采用酶联免疫吸附试验（ELISA）法对患者血清和腹腔液YKL-40水平进行检测,并进行统计学分析,同时分析EMS患者CA125与YKL-40水平的相关性关系。结果 ①EMS组患者血清和腹腔液CA125及YKL-40水平均高于对照组,并且差异均有统计学意义（P〈0.05）。②EMS组中Ⅲ～Ⅳ期患者血清和腹腔液CA125和YKL-40水平明显高于Ⅰ～Ⅱ期,并且差异均有统计学意义（P〈0.05）;EMS组中Ⅰ～Ⅱ期和Ⅲ～Ⅳ期患者的血清YKL-40水平分别高于对照组,EMS组中Ⅰ～Ⅱ期和Ⅲ～Ⅳ期患者的腹腔液中CA125、YKL-40水平分别高于对照组,并且差异均有统计学意义（P〈0.05）。③Spearman相关分析显示,EMS患者血清和腹腔液CA125和YKL-40水平间呈显著正相关关系（r=0.504,0.401;P〈0.01）。结论血清和腹腔液YKL-40水平有望成为EMS辅助诊断、提示其临床分期的检测指标。
Objective To investigate the expression levels of YKL-40 and CA125in the serum and peritoneal fluid of patients with endometriosis（EMS）,and discuss the relationship between YKL-40,and CA125.Methods From March 2012 to March 2014,a total of 50 cases with EMS were included into this study（EMS group）.At the same time,another 40 cases with benign uterus tumor were included into control group.There were no significant differences between two groups in the aspects of age,body mass index,the constituent ratio of algomenorrhea,the constituent ratio of infertility,and duration of infertility（P〈0.05）.The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Xuzhou Central Hospital,Affiliated Reproductive Medical Central of Southeast University.Informed consent was obtained from each patient.Serum and peritoneal fluid levels of YKL-40 in both two groups were detected by enzyme-linked immunosorbent assay（ELISA）,and serum and peritoneal fluid levels of CA125 were detected by electrochemiluminescence method.The relationship between CA125,YKL-40 and EMS were analyzed by Spearman correlation analysis.Results ①The serum and peritoneal fluid levels of CA125 and YKL-40 in EMS group were both significantly higher than those of control group,which with significant differences（P〈0.05）.②The serum and peritoneal fluid levels of CA125 and YKL-40 in stageⅢ-Ⅳ of EMS group were significantly higher than those of stageⅠ-Ⅱ,which with significant differences（P〈0.05）.The serum levels of YKL-40 in stageⅠ-Ⅱ,stageⅢ-Ⅳwere significantly higher than those of control group,and the peritoneal fluid levels of YKL-40,CA125 in stageⅠ-Ⅱ,stageⅢ-Ⅳwere significantly higher than those of control group,respectively,which both with significant differences（P〈0.05）.③Serum and peritoneal fluid levels of YKL-40 showed positive correlation with CA125（r=0.504,0.401;P〈0.01）by Spearman correlation analysis（P〈0.05）.Conclusions The serum and peritoneal fluid l
出处
《中华妇幼临床医学杂志（电子版）》
CAS
2015年第3期67-71,共5页
Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)
基金
河北省科学技术研究与发展计划项目(062761996)~~
