摘要
以红鳍东方鲀鱼为原料,从其皮中提取酸溶性胶原蛋白(ASC)和酶溶性胶原蛋白(PSC)。在此基础上,分析了红鳍东方鲀胶原蛋白的结构组成和纯度、热变性、氨基酸组成等基本理化性质。结果表明:红鳍东方鲀鱼皮中酸溶性胶原蛋白和酶溶性胶原蛋白纯度分别高达83.19%和85.00%;将所得胶原蛋白分别经紫外光谱分析、傅里叶红外光谱分析和SDS-PAGE电泳测定,确定酸溶性和酶溶性胶原蛋白符合Ⅰ型胶原蛋白的特征,三螺旋结构完整;经测定,ASC和PSC热变性温度分别为29.85和30.10℃。
The acid-solubilized collagen（ASC）and pepsin-solubilized collagen （PSC）extracted from skin of Takifugurubripes were studied on its physicochemical properties, including the structure, purity, thermal denaturation temperature and the amino acid composition, etc. The results show that the ASC and PSC had high purity of 83.19 % and 85.00 %, respectively. The analysis of UV spectra, FTIR spectra and SDS-PAGE of ASC and PSC show that the two kinds of collagen were proved to be proteinⅠwith integrate triple-helix structure. And the thermal denaturation temperatures of ASC and PSC were 29.85 and 30.10 ℃, respectively.
出处
《天津农学院学报》
CAS
2015年第2期6-10,共5页
Journal of Tianjin Agricultural University
基金
"十二五"国家科技支撑计划项目"黄渤海区鱼类工厂化健康养殖技术集成与示范"(2011BAD13B07)
国家星火计划重大项目"水产品健康养殖
加工与质量安全控制产业化示范"(2013GA610002)
天津市科技支撑计划重大项目"河鲀鱼良繁体系构建及大规模繁育"(12ZCDZNC05900)
关键词
红鳍东方鲀
鱼皮
胶原蛋白
理化性质
Takifugurubripes
skin
collagen
physicochemical characteristics
