摘要
为研究西南地区乌蒙乌骨鸡、云龙矮脚鸡、施甸鸡的遗传多样性及母系亲缘关系,通过PCR扩增、产物回收、测序的方法,获得3个地方鸡种81个个体的mt DNA D-loop区的部分序列,利用Clustal W、Dna SP5.10、MEGA4.0等生物信息学软件对测序结果进行分析。通过对81个个体mt DNA D-loop区731~795 bp的片段数据分析,并与原鸡属的mt DNA D-loop进行聚类,构建了单倍型及品种聚类图。结果显示,乌蒙乌骨鸡、云龙矮脚鸡、施甸鸡的单倍型多样性指数分别为0.712±0.068、0.895±0.048、0.731±0.074;乌蒙乌骨鸡、云龙矮脚鸡、施甸鸡具有较近的亲缘关系,推测3个地方鸡种起源于分布在越南北部、中国云南东南部、广西西南部、雷周半岛的徐闻、海南岛的Gallus gallus jabouillei亚种。
To investigate genetic diversity and maternal origin of Wumeng Black-bone, Yunlongaijiao and Shidian chicken breeds in the Southwest of China, PCR amplification, DNA collection and sequencing were used to obtain the partial sequence information of mitochondrial DNA（mtDNA）D-loop region from 81 samples of the three chicken breeds, then Clustal W, MEGAg.0, DnaSP5.10 were employed to analyze the sequences. The sequence information of 731 to 795 bp fragment of mtDNA was obtained from 81 samples and a haplotype network and a hierarchical cluster tree of chicken breeds were established. The haplotype diversity of Wumeng Black-bone, Yunlongaijiao and Shidian chicken were 0.712~0.068,0.895~0.048,0.731~O.074,respectively. Sequence analysis revealed little genetic differentiation among Wumeng Black-bone, Yunlongaijiao and Shidian chicken populations. Those maternal origins were presumed to be from Callus gallus jabouillei in Northern Vietnam, south-eastern Yunnan, south-western Guangxi, Xuwen on LeiZhou peninsula and Hainan Island.
出处
《中国家禽》
北大核心
2015年第11期19-22,共4页
China Poultry
基金
江苏省农业科技自主创新项目(CX(13)2038)
苏北科技专项资金(面上培育)项目(BN2014115)
