摘要
目的在妊娠期肝内胆汁淤积症(ICP)患者脐带血中检测维生素D的水平及维生素D受体(VDR)的表达情况,来阐述维生素D在ICP中的作用。方法纳入研究对象孕周相匹配的轻度ICP组孕妇45人,重度ICP组孕妇45人,正常组孕妇45人,三组孕妇脐血中维生素D浓度采用酶联免疫吸附试验(ELISA)法,脐血中VDR m RNA的表达水平采用实时荧光定量PCR法。结果 (1)脐血中VDR m RNA的表示水平:正常组>轻度ICP组>重度ICP组,两两比较差异均有统计学意义;(2)脐血中维生素D浓度:轻度ICP组与正常组比较无明显差异,两组均明显高于重度ICP组;(3)脐血中维生素D浓度与VDR m RNA的表示水平相关性:三组中均无明显相关性。结论 ICP患者脐血中存在维生素D缺乏或者是VDR表达的降低,可能与ICP的发病有一定的关系。
Objective： Testing the levels of the vitamin D and the expression of the vitamin D receptor （VDR） in the umbilical cord blood of patients with intrahepatic cholestasis of pregnancy （ICP） , so as to study the role of vitamin D in ICP. Methods： The objects of the study were 45 pregnant women with mild ICP, 45 with severe ICP and 45 normal pregnant women, who were the phase matching on gestational age. The levels vitamin D were detected by enzyme linked immunosorbent assay （ELISA） . The expression of the VDR were detected by real time fluorescence polymerase chain reaction （RT-PCR） . Results： （ 1 ） The levels of VDR expression in the umbilical cord blood： the normal group〉the mild ICP group〉the severe ICP group. （ 2 ） The levels of the vitamin D in the umbilical cord blood： no different in the mild ICP group and the normal group, the two groups were significantly higher than that of severe ICP group. （3） The correlation between the Vitamin D concentration and the VDR mRNA expression levels： no significant correlation in the three groups.Conclusions： There were vitamin D deficiency or the lower expression of VDR in the umbilical cord blood of patients with ICP, which was related to the onset of ICP.
出处
《中国优生与遗传杂志》
2015年第6期70-71,118,共3页
Chinese Journal of Birth Health & Heredity
基金
广州市花都区医疗卫生科研项目(编号14-HDWS-059)
