摘要
[目的]了解上海市2014年腹泻患者中弯曲菌感染现状,并分析弯曲菌的毒力基因及分子分型特征。[方法]采用膜过滤法对2014年上海市2 235例腹泻患者肛拭标本进行弯曲菌检测,并用常规生化试验和PCR方法鉴定分离菌株。采用PCR检测弯曲菌分离株的6种毒力相关基因,包括鞭毛蛋白基因fla A,细胞溶涨毒素cdt基因簇cdt A、cdt B、cdt C,p Vir质粒vir B同源性基因vir B11,外膜蛋白基因cad F。采用脉冲场凝胶电泳(PFGE)法对弯曲菌分离株进行分子分型。[结果]2 235例腹泻患者的肛拭标本中共检出弯曲菌43株,阳性率为1.9%。其中空肠弯曲菌占95.3%(41/43),结肠弯曲菌占4.7%(2/43)。毒力相关基因检测显示,100.0%(43/43)的弯曲菌菌株fla A基因和cad F基因阳性,93.0%(40/43)的弯曲菌菌株cdt A基因和cdt B基因阳性,88.4%(38/43)的弯曲菌菌株cdt C基因阳性,只有7.0%(3/43)的弯曲菌菌株vir B11基因阳性。43株弯曲菌经PFGE分型,共分为6个聚类。[结论]上海市腹泻患者中分离的弯曲菌普遍存在fla A和cad F基因,cdt A、cdt B、cdt C基因携带率高,vir B11携带率略低。弯曲菌分子分型呈多样化和复杂化特征,其引起的腹泻以散发为主。
[ Objective] To investigate the status quo of Campylobacter spp. infection in Shanghai and study its molecular characteristics and virulence and toxin genes. [ Methods ] Stool samples collected from diarrheal patients were cultured for bacterial pathogens using membrane filter method. The strains were identified by biochemical tests and PCR. PCR was applied to detect six virulence and toxin genes including flaA, cdtA, cdtB, cdtC, virB11, eadF. Pulsed - field gel electrophoresis { PFGE } was carried out for subtyping. [ Results ] A total of 43 Campylobacter spp. { 1.9% } were collected from 2 235 stool samples in Shanghai in 2014 including 41 Campylobacterjejuni isolates（95.3% ） and 2 Campylobacter coli isolates{4.7% ）. The data showed 100.0% （43/43） of the isolates were positive forflaA and cadF, and 93.0% { 40/43 ） of the isolates positive for cdtA and cdtB. And 88.4% { 38/43 ） of the isolates were posi- tive for cdtC. Only 7, 0% { 3/43 } of the isolates were positive for virB11. Using PFGE, 43 Campylobacter jejuni and Campylobacter coli strains were subtyped into 6 clusters. [ Conclusion] The genes offlaA and cadF are ubiquitous on Campylobacter spp. isolates. The distribution of cdt gene cluster in Campylobacter spp. is high, while that of virBll is low. The PFGE types of Campylobacter spp. isolated in Shanghai are of diversified and complicated features, which causes mainly sporatic diarrhea.
出处
《上海预防医学》
CAS
2015年第6期311-314,共4页
Shanghai Journal of Preventive Medicine
基金
上海市公共卫生重点学科卫生微生物学项目(12GWZX0801)
"十二五"国家科技重大专项(2013ZX10004216-001-004)
关键词
弯曲菌
毒力基因
分子分型
脉冲场凝胶电泳
Campylobacter spp
Virulence and toxin genes
Molecular type
Pulsed-field gel electrophoresis
