摘要
目的研究氯化锰对大鼠生精细胞细胞色素C（cytochrome-c，cyto—c）和支持细胞波形蛋白（vimentin，VM）表达的影响及对生精功能的抑制效应。方法将雄性SD大鼠随机分为空白对照、低剂量和高剂量组，8只／组。MnCl2组分别染锰4和6周，空白对照组给予等容生理盐水，给药途径均为腹腔注射，取睾丸采用免疫组织化学（SABC）法检测生精细胞cyto—c和支持细胞VM表达，测定睾丸脏器系数，取附睾检测精子数量和精子畸形率。结果与空白对照组比较，各染锰组生精细胞cyto—c阳性率由80％下降至60％，VM阳性率由50％下降至2％，睾丸脏器系数由1．2％下降至1％，精子数量由1．1×10^7／ml下降至0．2×10^7／ml，精子畸形率由28‰上升至64‰，且均呈一定的时间-效应关系和剂量-效应关系。各组大鼠精子数量与cyto—c和VM阳性率均呈正相关。结论锰可诱导大鼠cyto—c和VM表达，抑制生精功能。
[ Objective ] To determine the effect of manganese chloride on the expression of cytochrome-c （cyto-c） in spermatogenic epithelium and vimentin （VM） in sertoli ceils in rats, analyze the inhibitory on spermatogenic function. [ Methods ] Male SD rats were randomly divided into the control group, the low-dose group and the high-dose group, 8 rats in each group. The low-dose group and high-dose group were exposed to manganese for 4 and 6 weeks respectively, and the control group was given the normal saline. The administration route was injected into abdominal cavity. The expression of cyto-c in spermatogenic cells and VM in sertoli cells was investigated by immunohistochemistry （SABC）, and the organ coefficient of testis was tested. The sperm count and teratosperm rate were determined. [Results] Compared with the control groups, the positive rate of cyto-c in spermatogenic cells of two manganese exposure groups decreased from 80% to 60%, the positive rate of VM decreased from 50% to 2%, the organ coefficient of testis reduced from 1.2% to 1%, the sperm count decreased from 1.1×10^7/ml to 0.2×10^7/ml, the rate ofteratosperm increased from 28‰ to 64‰, and the time-effect relationship and dose-effect were observed in these indexes. The sperm count was positively correlated with the positive rate of cyto-c and VM in all groups. [ Conclusion ] IManganese can induce the expression of cyto-c and VM in rats, and inhibit the spermatogenie function.
出处
《职业与健康》
CAS
2015年第11期1476-1478,共3页
Occupation and Health
基金
遵义市科技项目[项目编号:遵市科合社字(200707号)]
遵义市红花岗区科技项目[项目编号:遵红科合社字(200622号)]
关键词
锰
大鼠
生精细胞
支持细胞
细胞色素C
波形蛋白
Manganese
Rat
Spermatogenic cell
Sertoli cell
Cytochrome-c （eyto-c）
Vimentin （VM）
