摘要
目的为了解济宁地区献血人群中人细小病毒B19(human parvovirus B19,简称HPV B19)感染情况,探讨血浆合并对其检出率的影响情况,降低临床输血风险提供一定依据。方法采用酶联免疫吸附法(ELISA)和实时荧光定量PCR(RTPCR)法对采集的960份献血员血液样本进行单人份及随机8人份血浆合并样本进行HPV B19-Ig G、Ig M抗体及DNA检测。结果单人份血浆与随机8人份血浆合并样本HPV B19 Ig G抗体、Ig M抗体阳性率分别为36.15%、5.94%、35.83%、12.50%,抗体阳性标本中单人份及血浆合并样本DNA阳性率分别为0.83%、5.00%。其中Ig M抗体阳性组B19 DNA检出率明显高于Ig G阳性组。结论献血人群中存在较高的HPV B19感染率,血浆合并后Ig M抗体及DNA检出率较高,未提示其传播风险降低,应对儿童、老年人、孕妇等免疫功能低下者选择性输注B19-Ig G抗体滴度较高的血液制品。
Objective To estimate the prevalence of parvovirus B19 infection （B19 for short） among blood doners in Jining and explore the effect of plasma mixing on HPV B19 detection rate, in order to provide a basis for reducing the risk of clinical blood transfusions. Methods Enzyme-linked immuno-sorbent （ELISA） and real-time fluorescence quantitative PCR （RT- PCR for short） assays were used for HPV B19 - IgG/IgM antibodies and DNA test from 960 blood samples and eight randomly selected mixed samples. Results The rates of positive BI9 IgG and IgM antibodies in the individual samples were 36. i5%, 5.94% respectively. For the eight randomly selected mixed samples 35.83%, 12. 50% respectively. Positive B19 - DNA rates for B19 - IgM positive sera were 0. 83% for individual samples and 5.00% for mixed samples. Moreover, B19 - IgM antibody and B19 - DNA positive rates of pooled plasma group were higher than those from the individual samples. Conclusions A high rate of B19 virus infection was found among blood donors. After plasma mixing, positive IgM antibody detection is increased but is insufficient to support that pooled plasma can reduce spreading of infection. Therefore, selected blood transfusion with high level B19 - IgG antibodies should be used for children, aged, pregnant women and other immuno-suppressive patients.
出处
《公共卫生与预防医学》
2015年第3期4-7,共4页
Journal of Public Health and Preventive Medicine
基金
济宁市医药卫生科技项目(2012jnjc09)
