摘要
目的探讨神经节苷脂(GM1)对人脑星形胶质瘤细胞U251内质网应激(ERS)相关凋亡的调控。方法用含不同浓度的GM1培养基培养U251细胞,MTT法检测细胞存活率,乳酸脱氢酶(LDH)检测细胞损伤,流式细胞术检测细胞凋亡率,Western blotting检测ERS凋亡相关蛋白CHOP及caspase-12表达变化。结果与对照组比较,GM1孵育48 h后,U251细胞存活率明显降低,且具有剂量依赖性(P<0.05);U251细胞培养基中LDH量明显增高,且具有剂量依赖性(P<0.05);GM1诱导U251细胞凋亡率明显增高,且具有剂量依赖性(P<0.05);GM1诱导细胞内CHOP及caspase-12的表达上调,且有剂量依赖性(P<0.05)。结论 GM1可诱导U251细胞ERS相关凋亡,为恶性脑胶质瘤的治疗提供新的方向。
Objective To investigate the effect of monosialotetrahexosy-1 ganglioside （ GM1 ） on endoplasmic reticulum stress （ERS）-associated cell apoptosis in malignant glioma U251 cells. Methods We used different con- centration of GM1 to treat U251 cells. The viability of cells was measured by MTY assay. The damage of cells was detected by lactate dehydrogenase （LDH）. The cell apoptosis was detected by flow cytometry. The ERS-related pro- tein expressions of CHOP and caspase-12 were assessed by Western blotting. Results After treatment of GM1 for 48 hours,the viability of U251 cells were significantly inhibited in dose-dependent manner （P 〈0. 05） ; and GM1 in- creased cell apoptosis rate in dose-dependent manner （ P 〈 0. 05 ）. Compared with the control group, the expression levels of CHOP and caspase-12 were significantly increased in the GM1 treatment groups （P 〈0.05 ）. Conclusion GM1 induces ERS-associated cell apoptosis in U251 cells,which provides a novel treatment for malignant glioma.
出处
《肿瘤基础与临床》
2015年第3期185-188,共4页
journal of basic and clinical oncology
基金
国家自然科学基金资助项目(编号:81301086)
关键词
细胞凋亡
内质网应激
恶性胶质瘤
U251细胞
神经节苷脂
cell apoptosis
endoplasmic reticulum stress
malignant glioma
U251 cells
monosialotetrahexosy-1 ganglioside
