摘要
在抗氧化活性追踪下,采用多种柱色谱与高压制备液相色谱等方法从忍冬叶50%甲醇提取物的乙酸乙酯萃取部分与正丁醇萃取部分分离鉴定了9个化合物,分别是5-O-咖啡酰基奎宁酸(1),绿原酸(2),4-O-咖啡酰基奎宁酸(3),木犀草素-7-O-[α-L-吡喃阿拉伯糖基(1→6)]-β-D-吡喃葡萄糖苷(4),木犀草苷(5),3,4-O-二咖啡酰奎宁酸(6),3,5-O-二咖啡酰奎宁酸(7),4,5-O-二咖啡酰奎宁酸(8)和木犀草素(9)。通过清除DPPH能力考察了这9个化合物抗氧化活性,其抗氧化能力由高到低依次为:化合物5>化合物9>抗坏血酸(对照)>化合物2>化合物8>化合物7>化合物6>化合物1>化合物3>化合物4;其中木犀草苷(IC500.018 18 g·L-1)的抗氧化活性最强,其次是木犀草素(IC500.023 6 g·L-1)与绿原酸(IC500.035 17g·L-1),且木犀草苷和木犀草素抗氧化活性均优于抗坏血酸(对照)(IC500.027 54 g·L-1)。试验结果为忍冬叶抗氧化产品的开发与利用奠定了基础。
Guided by the antioxidant activity, the EtOAc-soluble and n-butanol-soluble fractions of the 50% methanol extract of Lonicera japonica leaves were isolated and purified by various chromatorgraphie methods, and the structures were identified by spectral analysis and comparison to the data reported in literature. As a result, nine eomiumnds were obtained and identified as 5-O-caffeoylquinicaeid（1）, cldorogenieacid （ 2 ）, 4-O-caffeoylquinicacid （3）, luteolin-7-O-[ a-L-arobinopyranesyl- （ 1 →6 ） ] -β-D-glueopyranoside （4）, luteoloside（ 5 ）, 3,4-di-O-caffeoylquinic acid（ 6 ）, 3,5-di-O-eaffcoylquinic acid（ 7 ）, 4, 5-di-O-caffeoylquinic acid（ 8 ） and luteolin（9）. The antioxidant activity of the nine compounds were determined by using DPPH free radical scavenging method, and ascorbic acid was used as a positive control Their antioxidant activities from high to low were 5 〉 9 〉 2 〉8 〉 7 〉6 〉 1 〉 3 〉4. Among them, luteoleside（5 ） had the strongest antioxidant activity with an IC50 of 0. 018 18 g ·L^-1, and luteolin（ IC50 0. 023 6 g·L^-1） and ehlorogenieacid（ IC50 0. 035 17 g ·L^-1 ） ranks No. 2 and 3. Furthermore, the antioxidant activity of lutcoloside and luteolin were stronger than that of ascorhic acid（ IC50 0. 027 54 g·L^-1 ）. These results gave a basis for the further study and utilization of L japonica leaves.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2015年第12期2372-2377,共6页
China Journal of Chinese Materia Medica
关键词
忍冬叶
抗氧化活性
化学成分
定量测定
HPLC
Lonicera japonica leaves
anti-oxidant activity
chemical constituents
quantitative determination
HPLC
