摘要
在水温25~33℃下,将平均体质量74.8±1g的草鱼Ctenopharyngodon idellus放养在池塘网箱中,投喂以豆油、鱼油、氧化鱼油为饲料脂肪源的5组等氮等能半纯化饲料（豆油组6S、鱼油组6F、2%氧化鱼油组2OF、4%氧化鱼油组4OF,及6%氧化鱼油组6OF）,采用荧光定量PCR（q RT-PCR）方法,测定了草鱼肝胰脏中谷胱甘肽（GSH）/谷胱甘肽转移酶（GST）通路中GCLC、GSR、GSTPI、MGST1基因的表达活性及GSH的含量和超氧物歧化酶（SOD）的活性,研究了氧化鱼油对草鱼肝胰脏抗氧化防御能力的影响。72d的养殖结果显示：6F组GCLC的表达活性显著下调（P〈0.05）,其余各组间均无显著差异（P〉0.05）;各试验组GSR的表达活性均下调,6F和4OF组间显著下调（P〈0.05）;GSTPI的表达活性均显著下调（P〈0.05）,与饲料中（EPA＋DHA）含量呈线性负相关关系;除6F组外,其余各组MGST1的表达活性均较6S组显著下调（P〈0.05）,且MGST1的表达活性与饲料丙二醛（MDA）含量呈二项式关系,与6S组相比,其余各组肝胰脏中GSH含量及SOD活性均显著下调（P〈0.05）。氧化鱼油引起草鱼GSH/GST合成通路基因表达相适应,肝胰脏GSH合成相关基因和MGST1的表达活性下调,而GSTPI的表达活性增强。GSH/GSTs通路基因表达活性和GSH含量随饲料中氧化鱼油的增加呈梯度变化。
Juvenile grass carp Ctenopharyngodon idellus with body weight of （74.8± 1 ） g were reared in net cages disposed in a pond and fed five isonitrogenous and isoenergetic semi-purified diets containing soybean oil （6S）, fish oil （6F）, 2% oxidized fish oil （2OF）, 4% oxidized fish oil （4OF）, and 6% oxidized fish oil （6OF） at water temperature of 25-33℃ for 72 days to study the effects of dietary oxidized fish oil on oxidative stress pathway by detecting of GSH/GSTs pathway gene expression, GSH content, and SOD activity in hepatopancr eas of grass carp. The results showed that the expression of GCLC in 6OF group was significantly reduced （P〈0.05）, without significant differences among other groups （P〉0.05）. The expression of GSR was found to be reduced in each group, with significant difference between 6F group and 4OF group （P〈0.05）. There was significantly higher expression of GSTPI in experimental groups than that in 6S group （P〈0.05）, showing a negative relationship with dietary （EPA＋DHA） contents. The expres- sion of MGST1 was shown to be significantly reduced compared with that in 6S group （P〈0.05）. Except for 6G group, the expression of MGST1 was shown to be declined with a binomial relationship with MDA content. Compared with those in 6S group, GSH content and SOD activities in hepatopancreas were significantly reduced （P〈O.05）. In conclusion, oxidized fish oil leads to declined expres- sion of GSH synthesis related genes, GSTPI and increase in expression of MGST1 in hepatopancreas of grass carp, showing a gradient change in GSH/GSTs pathway gone expression and content of GSH in hcpatopancreas with increase in oxidized fish oil.
出处
《水产学杂志》
CAS
2015年第3期9-15,共7页
Chinese Journal of Fisheries
基金
国家自然基金(31172417)
关键词
草鱼
肝胰脏
氧化鱼油
GSH
抗氧化系统
grass carp Ctenopharyngodon idellus
hepatopancreas
oxidized fish oil
GSH
antioxidant system
