摘要
目的:探讨氧应激对肝星状细胞增殖的影响及还原型谷胱甘肽的抗氧化作用.方法:分别用不同浓度的次氮基三乙酸铁(Fe-NTa)培养大鼠肝星状细胞,在6,12,24和48h用四甲基偶氮唑盐法(MTT法)检测氧应激对肝星状细胞增殖的影响,检测丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性;并用不同浓度的还原型谷胱甘肽与Fe-NTa共同培养细胞,再检测SOD活性.结果:Fe-NTa作用12h与同一时间点的空白对照组比较,500和1000μmol/L组细胞增殖明显增多(A:0.369±0.124,0.485±0.101vs0.285±0.044,P<0.01),作用24和48h各浓度组与同一时间点的空白对照组比较差异显著(P<0.01).无Fe-NTa干预的空白对照组12,24和48h与同样处理的6h组比较,细胞增殖明显增多(A:0.285±0.044,0.253±0.033,0.278±0.037vs0.111±0.005,P<0.01),随着Fe-NTa剂量的增加,作用12,24和48h与同样处理的6h组比较,细胞增殖亦明显增多(P<0.01).200和500μmol/LFe-NTa组与对照组比较,SOD活力明显降低(156.95±21.17,100.92±10.02μkat/Lvs197.74±17.59μkat/L,P<0.01);MDA含量明显升高(1123±217,1549±182mmol/Lvs580±332mmol/L,P<0.01).预先加入GSH的各组与模型组(200μmol/LFe-NTa)比较,SOD活力明显升高(5.42±0.58,6.67±0.18,8.75±0.58μkat/Lvs2.25±0.35μkat/L,P<0.01).结论:氧应激促进HSC增殖有剂量和时间依赖性;且可导致脂质过氧化损伤,还原型谷胱甘肽有抗氧化作用,可对抗脂质过氧化损伤.
AIM： To explore the effects of oxidative stress on the proliferation of rat hepatic stellate cells and the antioxidation of reduced glutathione.
METHODS： Rat hepatic stellate cells were incubated with different concentrations of ferric nitrilotriacetic acid （Fe-NTa）. With 3-（4, 5-dimethyl- thiazol-2-yl）-2, 5-diphennylterazolium bromide （MTT） colorimetric assay, the effects of Fe-NTa on the proliferation of hepatic stellate cells at 6, 12, 24 and 48 h was detected, and malondialdehyde （MDA） contents and superoxide dismutase （SOD） activity were also detected. At the same time, hepatic stellate cells were incubated with different concentrations of reduced glutathione （0.5, 2.5, 10 mmol/L）, and MTT assay was used to SOD activity again.
RESULTS： In comparison with that in the blank control group at 12 h, the proliferation of hepatic stellate cells was significantly increased when the ferric nitrilotriacetic acid concentrations were 500 and 1000 μmol/L, respectively （A value： 0.369 ± 0.124, 0.485 ± 0.101 vs 0.285 ± 0.044, both P 〈 0.01）; the proliferation of cells incubated with different concentrations of FeNTa was also markedly increased at 24 and 48 h （P 〈 0.01）. The proliferation of hepatic stellate cells without Fe-NTa interference at 12, 24 and 48 h was also increased as compared with that at 6 h （A value： 0.285 ± 0.044, 0.253 ± 0.033, 0.278 ± 0.037 vs 0.111 ± 0.005, all P 〈 0.01）, while with the elevation of Fe-NTa concentration, the proliferation of hepatic stellate cells at 12, 24 and 48 h was markedly increased as compared with that at 6 h （P 〈 0.01）. In comparison with those in the control group, SOD activity significantly reduced （156.95 ± 21.17, 100.92 ± 10.02 μkat/L vs 197.74 ± 17.59 μkat/L, all P 〈 0.01） and MDA contents significantly increased （1123 ± 217, 1549 ± 182 mmol/L vs 580 ± 332 mmol/L, all P 〈 0.01） when the concentrations of Fe-NTa were 200 and 500 μmol/L. As compared with the model group （200 μmol/L
出处
《世界华人消化杂志》
CAS
北大核心
2006年第26期2596-2600,共5页
World Chinese Journal of Digestology
基金
上海市重点学科建设项目
No.Y0205
上海市科学技术委员会基金
No.054009618~~
关键词
氧应激
肝星状细胞
增殖
还原型谷胱甘肽
大鼠
Oxidatie stress
Hepatic stellate cell
Proliferation
Reduced glutathione
Rats
